Chinese cabbage has a high annual demand in China. However, clubroot disease caused by the infection of seriously affects its yield. Transcriptome analysis identified a root meristem growth factor 6 () as significantly up-regulated in Chinese cabbage roots infected with . Quantitative reverse-transcription polymerase chain reaction and hybridization analysis showed higher expression in susceptible materials than in resistant materials. After infection, expression was significantly up-regulated, especially in susceptible materials. Gene function analysis showed that the roots of mutant grew faster than the wild-type, and delayed the infection progress of . A Protein, nuclear transcription factor Y subunit C (BrNF-YC), was screened from yeast two-hybrid library of Chinese cabbage induced by , and verified to interact with BrRGF6 by yeast two-hybrid co-transfer. Yeast one-hybrid and β-Glucuronidase activity analysis showed that BrNF-YC could directly bind to and strongly activate the promoter of . Transgenic verification showed that or silenced Chinese cabbage significantly decreased the expression of , accelerated root development, and reduced incidence of clubroot disease. However, after overexpression of or , the phenotype showed a reverse trend. Therefore, silencing accelerated root growth and enhanced resistance to clubroot disease, which was regulated by BrNF-YC.
