The objectives of this experiment were to determine the AMEn content of different samples of corn gluten meal (CGM) and canola meal (CM) by a reference diet method and to develop prediction equations based on the chemical composition to estimate the AMEn value of CGM and CM in broilers. A total of 300 one-day-old male broiler chicks were randomly assigned to fifteen treatments (14 experimental diet and 1 reference diet) with 4 replicates of each with 5 birds per replicate. At first, birds were fed a starter diet from 0 to 10 d of age, and then, a grower diet from 11 to 23 d of age. To determine the AMEn content, the test diet consisted of 60% reference diet, 38% each test CGM or CM, and 2% minor ingredients. To adaptation, the broilers were fed experimental diets for 4 d, and then feces were collected on 28 d. The gross energy values and chemical compositions among the CGM and CM from different origins were significantly different. The AMEn values of the CGM samples varied from 3,123 to 3,918 kcal/kg, and for the CM, the range was from 1,578 to 2,109 kcal/kg. At the end of the experiment, data were analyzed with SPSS software, and a regression equation was obtained based on the chemical composition. The best equations were selected based on the standard of prediction and regression adjusted R. The equation, AMEn = 49.196 × CP + 80.87 × EE (SEP 180.99; adjusted R 0.97), was selected to predict the AMEn value of CGM, and the equation, AMEn = 631.55 × EE + 16.716 × CP (SEP 55.3; adjusted R 0.94), was selected to predict the AMEn value of CM.
