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Tropical medicine and infectious disease
Mar 13, 2023;8 (3):

Identification of Neotropical Mosquitoes by MALDI-TOF MS Profiling.

Monique Melo Costa, Amandine Guidez, Sébastien Briolant, Stanislas Talaga, Jean Issaly, Halima Naroua, Romuald Carinci, Pascal Gaborit, Anne Lavergne, Isabelle Dusfour, Jean-Bernard Duchemin, Lionel Almeras
Author Information
  1. Monique Melo Costa: Unité de Parasitologie et Entomologie, Département de Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 13005 Marseille, France.
  2. Amandine Guidez: Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97354 Cayenne, France.
  3. Sébastien Briolant: Unité de Parasitologie et Entomologie, Département de Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 13005 Marseille, France. ORCID
  4. Stanislas Talaga: Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97354 Cayenne, France.
  5. Jean Issaly: Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97354 Cayenne, France.
  6. Halima Naroua: Unité de Paludologie et d'Entomologie Médicale, CERMES, YN034, Niamey BP 10887, Niger.
  7. Romuald Carinci: Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97354 Cayenne, France.
  8. Pascal Gaborit: Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97354 Cayenne, France.
  9. Anne Lavergne: Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane, 97354 Cayenne, France.
  10. Isabelle Dusfour: Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97354 Cayenne, France. ORCID
  11. Jean-Bernard Duchemin: Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97354 Cayenne, France. ORCID
  12. Lionel Almeras: Unité de Parasitologie et Entomologie, Département de Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 13005 Marseille, France. ORCID
PMID: 36977169 DOI: 10.3390/tropicalmed8030168

Abstract

The mosquito (Diptera: Culicidae) fauna of French Guiana encompasses 242 species, of which nearly half of them belong to the genus . Whereas several species of are important vectors of arboviruses, only a limited number of studies focus on them due to the difficulties to morphologically identify field-caught females. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising method for the identification of mosquitoes. females collected in French Guiana were morphologically identified and dissected. Abdomens were used for molecular identification using the (cytochrome oxidase 1) gene. Legs and thorax of 169 specimens belonging to 13 species, (i.e., , , , , , , , , , , , and ) were then submitted to MALDI-TOF MS analysis. A high intra-species reproducibility and inter-species specificity of MS spectra for each mosquito body part tested were obtained. A corroboration of the specimen identification was revealed between MALDI-TOF MS, morphological and molecular results. MALDI-TOF MS protein profiling proves to be a suitable tool for identification of neotropical species and will permit the enhancement of knowledge on this highly diverse genus.

Keywords

Culex species French Guiana MALDI-TOF MS arbovirus monitoring neotropical mosquito proteomic tool

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Grants

  1. Grant no PDH-2-NBC 2-B-2201, MSProfileR project/Délégation Générale pour l'Armement (DGA)
  2. Conventions n° FIR/ARS/2014/91, n°FIR/ARS/2015/68, and Convention ARS/IPG 2021-2023 - Nov 26th 2021/Regional Health Agency of French Guiana
  3. Grant number GY0010695/European Union FEDER controle Programme
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