Gy��rgy Schneider: Department of Medical Microbiology and Immunology, Medical School, University of P��cs, Szigeti Str. 12, H-7624 P��cs, Hungary. ORCID
Dorina P��sztor: Department of Medical Microbiology and Immunology, Medical School, University of P��cs, Szigeti Str. 12, H-7624 P��cs, Hungary.
P��ter Szab��: Department of Geology and Meteorology, Faculty of Sciences, University of P��cs, Ifj��s��g Str. 6, H-7624 P��cs, Hungary.
L��szl�� K��r��si: Research Institute for Viticulture and Oenology, University of P��cs, P��zm��ny P. u. 4, H-7634 P��cs, Hungary. ORCID
Nandyala Siva Kishan: Centre for Research and Development, SRKR Engineering College, SRKR Marg, China Amiram, Bhimavaram 534204, India.
Penmetsa Appala Rama Krishna Raju: Department of Civil Engineering, SRKR Engineering College, SRKR Marg, China Amiram, Bhimavaram 534024, India.
Rajnish Kaur Calay: Institute for Building Energy and Materials Technology, Narvik Campus, UiT Norway's Arctic University, 8514 Narvik, Norway.
To develop efficient microbial fuel cell systems for green energy production using different waste products, establishing characterised bacterial consortia is necessary. In this study, bacteria with electrogenic potentials were isolated from mud samples and examined to determine biofilm-formation capacities and macromolecule degradation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identifications have revealed that isolates represented 18 known and 4 unknown genuses. They all had the capacities to reduce the Reactive Black 5 stain in the agar medium, and 48 of them were positive in the wolfram nanorod reduction assay. The isolates formed biofilm to different extents on the surfaces of both adhesive and non-adhesive 96-well polystyrene plates and glass. Scanning electron microscopy images revealed the different adhesion potentials of isolates to the surface of carbon tissue fibres. Eight of them (15%) were able to form massive amounts of biofilm in three days at 23 ��C. A total of 70% of the isolates produced proteases, while lipase and amylase production was lower, at 38% and 27% respectively. All of the macromolecule-degrading enzymes were produced by 11 isolates, and two isolates of them had the capacity to form a strong biofilm on the carbon tissue one of the most used anodic materials in MFC systems. This study discusses the potential of the isolates for future MFC development applications.
