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Probiotics and antimicrobial proteins
Apr, 2024;16 (2): 636-648.

Effect of Lactobacillus Rhamnosus GG for Regulation of Inflammatory Response in Radiation-Induced Enteritis.

Sung Uk Lee, Bum-Sup Jang, Yi Rang Na, Sun Hwa Lee, Sunwoo Han, Ji Hyun Chang, Hak Jae Kim
Author Information
  1. Sung Uk Lee: Department of Radiation Oncology, Seoul National University College of Medicine, Seoul, South Korea.
  2. Bum-Sup Jang: Department of Radiation Oncology, Seoul National University College of Medicine, Seoul, South Korea.
  3. Yi Rang Na: Transdisciplinary Department of Medicine and Advanced Technology, Seoul National University Hospital, Seoul, South Korea.
  4. Sun Hwa Lee: Department of Radiation Oncology, Seoul National University Hospital, Seoul, South Korea.
  5. Sunwoo Han: Transdisciplinary Department of Medicine and Advanced Technology, Seoul National University Hospital, Seoul, South Korea.
  6. Ji Hyun Chang: Department of Radiation Oncology, Seoul National University College of Medicine, Seoul, South Korea. jh.chang@snu.ac.kr.
  7. Hak Jae Kim: Department of Radiation Oncology, Seoul National University College of Medicine, Seoul, South Korea. khjae@snu.ac.kr.
PMID: 37072632 DOI: 10.1007/s12602-023-10071-9

Abstract

The purpose of this study was to investigate the role of Lactobacillus rhamnosus GG (LGG) probiotics in radiation enteritis using in vivo mice. A total of 40 mice were randomly assigned to four groups: control, probiotics, radiotherapy (RT), and RT + probiotics. For the group of probiotics, 0.2 mL of solution that contained 1.0 × 10 colony-forming units (CFU) of LGG was used and orally administered daily until sacrifice. For RT, a single dose of 14 Gy was administered using a 6 mega-voltage photon beam to the abdominopelvic area. Mice were sacrifice at day 4 (S1) and day 7 (S2) after RT. Their jejunum, colon, and stool were collected. A multiplex cytokine assay and 16 s ribosomal RNA amplicon sequencing were then performed. Regarding cytokine concentrations in tissues, pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin-6 and monocyte chemotactic protein-1, showed significantly decreased protein levels in colon tissues of the RT + probiotics group than in the RT alone group (all p < 0.05). As for comparing microbial abundance through alpha-diversity and beta-diversity, no significant differences were observed between the RT + probiotics and RT alone groups, except for an increase in alpha-diversity in the stool of the RT + probiotics group. Upon analysis of differential microbes based on treatment, the dominance of anti-inflammatory-related microbes, such as Porphyromonadaceae, Bacteroides acidifaciens, and Ruminococcus, was observed in the jejunum, colon, and stool of the RT + probiotics group. With regard to predicted metabolic pathway abundances, the pathways associated with anti-inflammatory processes, such as biosynthesis of pyrimidine nucleotides, peptidoglycans, tryptophan, adenosylcobalamin, and propionate, were differentially identified in the RT + probiotics group compared to the RT alone group. Protective effects of probiotics on radiation enteritis were potentially derived from dominant anti-inflammation-related microbes and metabolites.

Keywords

Enteritis Microbiota Probiotics Radiotherapy Sequence analysis

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Grants

  1. 2019R1A2C1002071/Ministry of Education

MeSH Term

Mice
Animals
Lacticaseibacillus rhamnosus
Cytokines
Enteritis
Interleukin-6
Anti-Inflammatory Agents
Probiotics

Chemicals

Cytokines
Interleukin-6
Anti-Inflammatory Agents
Journal Article
Article has an altmetric score of 1

Word Cloud

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