A multifaceted biomimetic periosteum with a lamellar architecture and osteogenic/angiogenic dual bioactivities.
Yonggang Zhao, Yang Xiong, Jingchuan Zheng, Wenyao Kongling, Junlin Chen, Chengli Li, Peilun Hu, Shuhui Yang, Xiumei Wang
Author Information
Yonggang Zhao: State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials of Ministry of Education, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. wxm@mail.tsinghua.edu.cn.
Yang Xiong: State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials of Ministry of Education, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. wxm@mail.tsinghua.edu.cn.
Jingchuan Zheng: State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials of Ministry of Education, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. wxm@mail.tsinghua.edu.cn.
Wenyao Kongling: School of Stomatology, Dalian Medical University, Dalian, China.
Junlin Chen: State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials of Ministry of Education, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. wxm@mail.tsinghua.edu.cn.
Chengli Li: School of Clinical Medicine, Tsinghua University, Department of Orthopaedics, Beijing Tsinghua Changgung Hospital, Beijing, 102218, China.
Peilun Hu: School of Clinical Medicine, Tsinghua University, Department of Orthopaedics, Beijing Tsinghua Changgung Hospital, Beijing, 102218, China.
Shuhui Yang: State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials of Ministry of Education, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. wxm@mail.tsinghua.edu.cn.
Xiumei Wang: State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials of Ministry of Education, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. wxm@mail.tsinghua.edu.cn. ORCID
An artificial periosteum has emerged as an encouraging candidate for bone defect repair. Currently, it remains a great challenge to develop a multifaceted biomimetic periosteum integrating multifunctional features of bioactivities and unique mechanical properties. Here, we successfully fabricated an artificial periosteum (AP) composed of hierarchically assembled Mg-doped mineralized collagen microfibrils with a biomimetically rotated lamellar structure a "multiscale cascade regulation" strategy combining multiple techniques such as molecular self-assembly, electrospinning, and pressure-driven fusion from molecular to macroscopic levels. The AP has excellent mechanical properties with an ultimate strength and a tensile modulus of 15.9 MPa and 1.1 GPa, respectively. The involvement of Mg-doped nano-hydroxyapatite endowed the AP with good osteogenic and angiogenic activities to promote osteogenic differentiation of bone marrow mesenchymal stem cells and human umbilical vein endothelial cell differentiation into capillary-like structures . In addition, the results of evaluations in a rat cranial bone defect model including micro-CT morphology, histological staining, and immunohistochemical analysis showed that Mg-doped mineralized collagen-based AP (MgMC@AP) significantly facilitated cranial bone regeneration and fast vascularization. Our findings suggest that the AP mimicked the composition, lamellar structure, mechanical properties, and biological activities of natural periosteum/lamellae, showing great promise for bone tissue regeneration.
