Ribosomal DNA Sequence-Based Taxonomy and Antimicrobial Activity of spp. (Dinophyceae) from Mauritius Coastal Waters, South-West Indian Ocean.

Prakash Mussai, Jacob Larsen, Abdulwahed Fahad Alrefaei, Rajesh Jeewon
Author Information
  1. Prakash Mussai: Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Reduit 80837, Mauritius.
  2. Jacob Larsen: IOC Science and Communication Centre on Harmful Algae, Biological Institute, University of Copenhagen, Universitetsparken 4, DK-2100 Copenhagen, Denmark.
  3. Abdulwahed Fahad Alrefaei: Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. ORCID
  4. Rajesh Jeewon: Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Reduit 80837, Mauritius. ORCID

Abstract

Microalgae are unicellular organisms and commonly present in the euphotic zone of marine ecosystems. From the western coast of Mauritius, three strains of species were isolated from macrophytes and cultured under standard laboratory conditions. Morphologies were examined by light, fluorescence, and scanning electron microscopy, and phylogenetic analyses were based on partial large subunit LSU rDNA (D1-D2) and ITS1-5.8S-ITS2 (ITS) regions. Three species, including the complex, , and complex, were identified. The antimicrobial activities were assayed against potential human pathogenic bacterial strains. The highest zone of inhibition was recorded for intracellular and extracellular protein extracts of against . The polysaccharide extracts of the complex had a higher zone of inhibition (24 ± 0.4 mm) against MRSA at a minimum concentration of 0.625 μg/mL. The extracts from the three species had different levels of activity against the pathogens used, and this can be of scientific interest in the search for antibiotics from natural marine sources.

Keywords

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MeSH Term

Humans
DNA, Ribosomal
Phylogeny
Ecosystem
Base Sequence
Indian Ocean
Mauritius
Anti-Infective Agents
Dinoflagellida

Chemicals

DNA, Ribosomal
Anti-Infective Agents

Word Cloud

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