Mapping of QTLs Associated with Biological Nitrogen Fixation Traits in Peanuts ( L.) Using an Interspecific Population Derived from the Cross between the Cultivated Species and Its Wild Ancestors.
Darius T Nzepang, Djamel Gully, Joël R Nguepjop, Arlette Zaiya Zazou, Hodo-Abalo Tossim, Aissatou Sambou, Jean-François Rami, Valerie Hocher, Saliou Fall, Sergio Svistoonoff, Daniel Fonceka
Author Information
Darius T Nzepang: Centre d'Etudes Régional pour l'Amélioration de l'Adaptation à la Sécheresse, CERAAS-Route de Khombole, Thiès BP 3320, Senegal. ORCID
Djamel Gully: PHIM Plant Health Institute, Univ Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, France. ORCID
Joël R Nguepjop: Centre d'Etudes Régional pour l'Amélioration de l'Adaptation à la Sécheresse, CERAAS-Route de Khombole, Thiès BP 3320, Senegal.
Arlette Zaiya Zazou: Institute of Agricultural Research for Development (IRAD) (IRAD), Maroua, Cameroon.
Hodo-Abalo Tossim: Centre d'Etudes Régional pour l'Amélioration de l'Adaptation à la Sécheresse, CERAAS-Route de Khombole, Thiès BP 3320, Senegal.
Aissatou Sambou: Centre d'Etudes Régional pour l'Amélioration de l'Adaptation à la Sécheresse, CERAAS-Route de Khombole, Thiès BP 3320, Senegal.
Jean-François Rami: Dispositif de Recherche et de Formation en Partenariat, Innovation et Amélioration Variétale en Afrique de l'Ouest (IAVAO), CERAAS Route de Khombole, Thiès BP 3320, Senegal.
Valerie Hocher: PHIM Plant Health Institute, Univ Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, France.
Saliou Fall: Laboratoire Commun de Microbiologie (LCM) (IRD/ISRA/UCAD), Centre de Recherche de Bel Air, Dakar BP 1386, Senegal.
Sergio Svistoonoff: PHIM Plant Health Institute, Univ Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, France. ORCID
Daniel Fonceka: Centre d'Etudes Régional pour l'Amélioration de l'Adaptation à la Sécheresse, CERAAS-Route de Khombole, Thiès BP 3320, Senegal. ORCID
Peanuts ( L.) are an allotetraploid grain legume mainly cultivated by poor farmers in Africa, in degraded soil and with low input systems. Further understanding nodulation genetic mechanisms could be a relevant option to facilitate the improvement of yield and lift up soil without synthetic fertilizers. We used a subset of 83 chromosome segment substitution lines (CSSLs) derived from the cross between a wild synthetic tetraploid AiAd ( × ) and the cultivated variety Fleur11, and evaluated them for traits related to BNF under shade-house conditions. Three treatments were tested: without nitrogen; with nitrogen; and without nitrogen, but with added0 strain ISRA400. The leaf chlorophyll content and total biomass were used as surrogate traits for BNF. We found significant variations for both traits specially linked to BNF, and four QTLs (quantitative trait loci) were consistently mapped. At all QTLs, the wild alleles decreased the value of the trait, indicating a negative effect on BNF. A detailed characterization of the lines carrying those QTLs in controlled conditions showed that the QTLs affected the nitrogen fixation efficiency, nodule colonization, and development. Our results provide new insights into peanut nodulation mechanisms and could be used to target BNF traits in peanut breeding programs.
