Protein Interaction Network Analysis to Investigate Stress Response, Virulence, and Antibiotic Resistance Mechanisms in .

Robert Hanes, Fangyuan Zhang, Zuyi Huang
Author Information
  1. Robert Hanes: Department of Chemical Engineering, Villanova University, Villanova, PA 19085, USA.
  2. Fangyuan Zhang: Department of Chemical Engineering, Villanova University, Villanova, PA 19085, USA. ORCID
  3. Zuyi Huang: Department of Chemical Engineering, Villanova University, Villanova, PA 19085, USA.

Abstract

is a deadly and costly foodborne pathogen that has a high fatality rate in the elderly, pregnant women, and people with weakened immunity. It can survive under various stress conditions and is a significant concern for the food industry. In this work, a data analysis approach was developed with existing tools and databases and used to create individual and combined protein interaction networks to study stress response, virulence, and antimicrobial resistance and their interaction with . The networks were analyzed, and 28 key proteins were identified that may serve as potential targets for new strategies to combat . Five of the twenty-eight proteins (i.e., sigB, flaA, cheA, cheY, and lmo0693) represent the most promising targets because they are highly interconnected within the combined network. The results of this study provide a new set of targets for future work to identify new strategies to improve food preservation methods and treatments for .

Keywords

References

  1. Antibiotics (Basel). 2022 Apr 14;11(4): [PMID: 35453279]
  2. Food Res Int. 2023 Mar;165:112487 [PMID: 36869448]
  3. J Bacteriol. 2003 Oct;185(19):5722-34 [PMID: 13129943]
  4. Int J Environ Res Public Health. 2022 May 01;19(9): [PMID: 35564901]
  5. Infect Immun. 2005 Sep;73(9):5530-9 [PMID: 16113269]
  6. Trends Microbiol. 2008 Aug;16(8):388-96 [PMID: 18619843]
  7. Antimicrob Agents Chemother. 2012 May;56(5):2696-704 [PMID: 22354291]
  8. Methods Mol Biol. 2012;850:483-94 [PMID: 22307715]
  9. Nature. 2000 May 18;405(6784):299-304 [PMID: 10830951]
  10. Antimicrob Agents Chemother. 1999 Sep;43(9):2103-8 [PMID: 10471548]
  11. PLoS Genet. 2018 Sep 4;14(9):e1007525 [PMID: 30180166]
  12. Semin Cell Dev Biol. 2015 Oct;46:91-103 [PMID: 26541483]
  13. Microbiol Spectr. 2018 Jul;6(4): [PMID: 30027884]
  14. Iran J Microbiol. 2020 Aug;12(4):305-312 [PMID: 32994901]
  15. BMC Syst Biol. 2014;8 Suppl 4:S11 [PMID: 25521941]
  16. Wien Med Wochenschr. 2019 Feb;169(Suppl 1):25-30 [PMID: 30623278]
  17. Appl Environ Microbiol. 2007 Oct;73(19):6078-88 [PMID: 17704270]
  18. Infect Immun. 2006 May;74(5):2505-12 [PMID: 16622185]
  19. Front Microbiol. 2018 Nov 13;9:2700 [PMID: 30555426]
  20. Cell Chem Biol. 2016 Mar 17;23(3):404-14 [PMID: 26991105]
  21. Vaccines (Basel). 2020 Dec 17;8(4): [PMID: 33348708]
  22. Annu Rev Microbiol. 2007;61:215-36 [PMID: 18035607]
  23. J Cell Physiol. 2021 Jan;236(1):468-479 [PMID: 32542649]
  24. J Appl Microbiol. 2016 Feb;120(2):251-65 [PMID: 26509460]
  25. Infect Genet Evol. 2018 Nov;65:127-130 [PMID: 30053641]
  26. Infect Immun. 2004 Jun;72(6):3237-44 [PMID: 15155625]
  27. Appl Environ Microbiol. 2007 Dec;73(24):7967-80 [PMID: 17933929]
  28. Eur J Clin Microbiol Infect Dis. 2015 May;34(5):877-86 [PMID: 25630538]
  29. Antibiotics (Basel). 2021 Oct 19;10(10): [PMID: 34680854]
  30. Int J Environ Res Public Health. 2020 Jan 10;17(2): [PMID: 31936874]
  31. J Bacteriol. 2020 Mar 11;202(7): [PMID: 31964697]
  32. J Proteomics. 2020 Aug 30;226:103906 [PMID: 32707233]
  33. Appl Environ Microbiol. 2015 Aug 15;81(16):5350-62 [PMID: 26025900]
  34. ACS Omega. 2020 Mar 23;5(13):7537-7544 [PMID: 32280897]
  35. Curr Microbiol. 2003 Jun;46(6):461-6 [PMID: 12732955]
  36. BMC Bioinformatics. 2003 Jan 13;4:2 [PMID: 12525261]

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