Easy, Flexible and Standardizable Anti-Nascent Biofilm Activity Assay to Assess Implant Materials.
Jérome Vanheuverzwijn, Eloise-Eliane Maillard, Amal Mahat, Lee Fowler, Daniel Monteyne, Leïla Bonnaud, Nicolas Landercy, Axel Hemberg, Ana Janković, Franck Meyer, Vesna Mišković-Stanković, Milena Stevanović, Codruta Mirica, David Pérez-Morga, Reto Luginbuehl, Christèle Combes, Gabriel Furtos, Véronique Fontaine
Author Information
Jérome Vanheuverzwijn: Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium.
Eloise-Eliane Maillard: Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium.
Amal Mahat: Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium. ORCID
Lee Fowler: Applied Materials Science, The Ångström Laboratory, Department of Engineering Sciences, Uppsala University, P.O. Box 534, 75121 Uppsala, Sweden.
Daniel Monteyne: Laboratoire de Parasitologie Moléculaire, Faculté des Sciences & CMMI, Université Libre de Bruxelles (ULB), CP 300. Rue Prof. Jeener & Brachet, 12, 6041 Gosselies, Belgium.
Leïla Bonnaud: Center of Innovation and Research in Materials and Polymers, Materia Nova Research Center & University of Mons, 7000 Mons, Belgium. ORCID
Nicolas Landercy: Center of Innovation and Research in Materials and Polymers, Materia Nova Research Center & University of Mons, 7000 Mons, Belgium.
Axel Hemberg: Center of Innovation and Research in Materials and Polymers, Materia Nova Research Center & University of Mons, 7000 Mons, Belgium.
Ana Janković: Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Franck Meyer: Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium. ORCID
Vesna Mišković-Stanković: Faculty of Ecology and Environmental Protection, University Union-Nikola Tesla, Cara Dusana 62-64, 11158 Belgrade, Serbia. ORCID
Milena Stevanović: Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia. ORCID
Codruta Mirica: Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Victor Babes Street 15, 400012 Cluj-Napoca, Romania.
David Pérez-Morga: Laboratoire de Parasitologie Moléculaire, Faculté des Sciences & CMMI, Université Libre de Bruxelles (ULB), CP 300. Rue Prof. Jeener & Brachet, 12, 6041 Gosselies, Belgium.
Reto Luginbuehl: Department of Biomedical Material Research, University of Bern, 3008 Bern, Switzerland. ORCID
Christèle Combes: Centre Inter-Universitaire de Recherche et d'Ingénierie des Matériaux, CIRIMAT, Toulouse INP, Université Toulouse 3 Paul Sabatier, CNRS, Université de Toulouse, 4 allée Emile Monso, BP44362, CEDEX 4, 31030 Toulouse, France. ORCID
Gabriel Furtos: Department of Dental Materials, Institute of Research in Chemistry, Babes-Bolyai University-Raluca Ripan, Fantanele Street 30, 400294 Cluj-Napoca, Romania. ORCID
Véronique Fontaine: Microbiology, Bioorganic and Macromolecular Chemistry Unit, Faculty of Pharmacy, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium. ORCID
Medical implants have improved the quality of life of many patients. However, surgical intervention may eventually lead to implant microbial contamination. The aims of this research were to develop an easy, robust, quantitative assay to assess surface antimicrobial activities, especially the anti-nascent biofilm activity, and to identify control surfaces, allowing for international comparisons. Using new antimicrobial assays to assess the inhibition of nascent biofilm during persistent contact or after transient contact with bacteria, we show that the 5 cent Euro coin or other metal-based antibacterial coins can be used as positive controls, as more than 4 log reduction on bacterial survival was observed when using either or as targets. The methods and controls described here could be useful to develop an easy, flexible and standardizable assay to assess relevant antimicrobial activities of new implant materials developed by industries and academics.
