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May 15, 2023;14 (5):

Evaluation of the Antibacterial Activity of the Peptide Fractions Extracted from the Hemolymph of (Diptera: Stratiomyidae).

Carmen Scieuzo, Fabiana Giglio, Roberta Rinaldi, Marilena E Lekka, Flora Cozzolino, Vittoria Monaco, Maria Monti, Rosanna Salvia, Patrizia Falabella
Author Information
  1. Carmen Scieuzo: Department of Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy. ORCID
  2. Fabiana Giglio: Department of Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy. ORCID
  3. Roberta Rinaldi: Department of Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy. ORCID
  4. Marilena E Lekka: Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece. ORCID
  5. Flora Cozzolino: Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy. ORCID
  6. Vittoria Monaco: Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy. ORCID
  7. Maria Monti: Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy. ORCID
  8. Rosanna Salvia: Department of Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy. ORCID
  9. Patrizia Falabella: Department of Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy. ORCID
PMID: 37233092 DOI: 10.3390/insects14050464

Abstract

Antimicrobial peptides (AMPs) are a chemically and structurally heterogeneous family of molecules produced by a large variety of living organisms, whose expression is predominant in the sites most exposed to microbial invasion. One of the richest natural sources of AMPs is insects which, over the course of their very long evolutionary history, have adapted to numerous and different habitats by developing a powerful innate immune system that has allowed them to survive but also to assert themselves in the new environment. Recently, due to the increase in antibiotic-resistant bacterial strains, interest in AMPs has risen. In this work, we detected AMPs in the hemolymph of (Diptera, Stratiomyidae) larvae, following infection with (Gram negative) or (Gram positive) and from uninfected larvae. Peptide component, isolated via organic solvent precipitation, was analyzed by microbiological techniques. Subsequent mass spectrometry analysis allowed us to specifically identify peptides expressed in basal condition and peptides differentially expressed after bacterial challenge. We identified 33 AMPs in all the analyzed samples, of which 13 are specifically stimulated by Gram negative and/or Gram positive bacterial challenge. AMPs mostly expressed after bacterial challenge could be responsible for a more specific activity.

Keywords

AMPs Escherichia coli Micrococcus flavus antibiotic resistance black soldier fly

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Grants

  1. PRIN2017, protocol Prot. 2017AHTCK7/Italian Ministry University and Research
  2. PO FESR, BASILICATA 2014-2020 "AAA: SAFE SOS" D.D. 12AF.2020/D.01255-9/11/2020/Basilicata Region
Journal Article
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Word Cloud

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