An Exploratory Study of the Metabolite Profiling from Pesticides Exposed Workers.
Daniela Magalhães Nolasco, Michele P R Mendes, Luiz Paulo de Aguiar Marciano, Luiz Filipe Costa, Adriana Nori De Macedo, Isarita Martins Sakakibara, Alessandra Cristina Pupin Silvério, Maria José N Paiva, Leiliane C André
Author Information
Daniela Magalhães Nolasco: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil.
Michele P R Mendes: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil.
Luiz Paulo de Aguiar Marciano: Toxicants and Drugs Analysis Laboratory, Faculty of Pharmacy, Federal University of Alfenas (UNIFAL), Alfenas 37130-001, MG, Brazil. ORCID
Luiz Filipe Costa: Toxicants and Drugs Analysis Laboratory, Faculty of Pharmacy, Federal University of Alfenas (UNIFAL), Alfenas 37130-001, MG, Brazil. ORCID
Adriana Nori De Macedo: Chemistry Department, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil. ORCID
Isarita Martins Sakakibara: Toxicants and Drugs Analysis Laboratory, Faculty of Pharmacy, Federal University of Alfenas (UNIFAL), Alfenas 37130-001, MG, Brazil. ORCID
Alessandra Cristina Pupin Silvério: Faculty of Pharmacy, Professor Édson Antônio Vellano University (UNIFENAS), Alfenas 37132-440, MG, Brazil.
Maria José N Paiva: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil.
Leiliane C André: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil. ORCID
Pesticides constitute a category of chemical products intended specifically for the control and mitigation of pests. With their constant increase in use, the risk to human health and the environment has increased proportionally due to occupational and environmental exposure to these compounds. The use of these chemicals is associated with several toxic effects related to acute and chronic toxicity, such as infertility, hormonal disorders and cancer. The present work aimed to study the metabolic profile of individuals occupationally exposed to pesticides, using a metabolomics tool to identify potential new biomarkers. Metabolomics analysis was carried out on plasma and urine samples from individuals exposed and non-exposed occupationally, using liquid chromatography coupled with mass spectrometry (UPLC-MS). Non-targeted metabolomics analysis, using principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) or partial least squares discriminant orthogonal analysis (OPLS-DA), demonstrated good separation of the samples and identified 21 discriminating metabolites in plasma and 17 in urine. The analysis of the ROC curve indicated the compounds with the greatest potential for biomarkers. Comprehensive analysis of the metabolic pathways influenced by exposure to pesticides revealed alterations, mainly in lipid and amino acid metabolism. This study indicates that the use of metabolomics provides important information about complex biological responses.
