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Bioengineering (Basel, Switzerland)
May 08, 2023;10 (5):

Efficient Bioremediation of Petroleum-Contaminated Soil by Immobilized Bacterial Agent of W33.

Yong Yang, Wanze Zhang, Zhanwei Zhang, Ting Yang, Zhuo Xu, Chuanbo Zhang, Bing Guo, Wenyu Lu
Author Information
  1. Yong Yang: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
  2. Wanze Zhang: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
  3. Zhanwei Zhang: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
  4. Ting Yang: China Offshore Environmental Service Ltd., Tianjin 300457, China.
  5. Zhuo Xu: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
  6. Chuanbo Zhang: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. ORCID
  7. Bing Guo: China Offshore Environmental Service Ltd., Tianjin 300457, China.
  8. Wenyu Lu: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. ORCID
PMID: 37237630 DOI: 10.3390/bioengineering10050561

Abstract

In this article, we report a method for preparing an immobilized bacterial agent of petroleum-degrading bacteria W33 by combining high-density fermentation and bacterial immobilization technology and testing its bioremediation effect on petroleum-contaminated soil. After determining the optimal combination of MgCl, CaCl concentration, and culture time in the fermentation conditions by conducting a response surface analysis, the cell concentration reached 7.48 × 10 CFU/mL by 5 L fed-batch fermentation. The W33-vermiculite-powder-immobilized bacterial agent mixed with sophorolipids and rhamnolipids in a weight ratio of 9:10 was used for the bioremediation of petroleum-contaminated soil. After 45 days of microbial degradation, 56.3% of the petroleum in the soil with 20,000 mg/kg petroleum content was degraded, and the average degradation rate reached 250.2 mg/kg/d.

Keywords

Gordonia alkanivorans W33 bioremediation microbial immobilization petroleum-contaminated soil

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Grants

  1. 2018GKF-0785/CNOOC Environmental Protection Service Co., Ltd.
Journal Article

Word Cloud

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