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Virulence
12, 2023;14 (1): 2224642.

Genomic characterization, transcriptome analysis, and pathogenicity of the Nipah virus (Indian isolate).

Sreelekshmy Mohandas, Anita Shete, Prasad Sarkale, Abhinendra Kumar, Chandrasekhar Mote, Pragya Yadav
Author Information
  1. Sreelekshmy Mohandas: Maximum Containment Facility, Indian Council of Medical Research-National Institute of Virology, Pune, Maharashtra, India.
  2. Anita Shete: Maximum Containment Facility, Indian Council of Medical Research-National Institute of Virology, Pune, Maharashtra, India.
  3. Prasad Sarkale: Maximum Containment Facility, Indian Council of Medical Research-National Institute of Virology, Pune, Maharashtra, India.
  4. Abhinendra Kumar: Maximum Containment Facility, Indian Council of Medical Research-National Institute of Virology, Pune, Maharashtra, India.
  5. Chandrasekhar Mote: Department of Veterinary Pathology, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Maharashtra, India.
  6. Pragya Yadav: Maximum Containment Facility, Indian Council of Medical Research-National Institute of Virology, Pune, Maharashtra, India.
PMID: 37312405 DOI: 10.1080/21505594.2023.2224642

Abstract

Nipah virus (NiV) is a high-risk pathogen which can cause fatal infections in humans. The Indian isolate from the 2018 outbreak in the Kerala state of India showed ~ 4% nucleotide and amino acid difference in comparison to the Bangladesh strains of NiV and the substitutions observed were mostly not present in the region of any functional significance except for the phosphoprotein gene. The differential expression of viral genes was observed following infection in Vero (ATCC® CCL-81™) and BHK-21 cells. Intraperitoneal infection in the 10-12-week-old, Syrian hamster model induced dose dependant multisystemic disease characterized by prominent vascular lesions in lungs, brain, kidney and extra vascular lesions in brain and lungs. Congestion, haemorrhages, inflammatory cell infiltration, thrombosis and rarely endothelial syncitial cell formation were seen in the blood vessels. Intranasal infection resulted in respiratory tract infection characterised by pneumonia. The model showed disease characteristics resembling the human NiV infection except that of myocarditis similar to that reported by NiV-Malaysia and NiV-Bangladesh isolates in hamster model. The variation observed in the genome of the Indian isolate at the amino acid levels should be explored further for any functional significance.

Keywords

Indian isolate Nipah virus characterization hamster pathogenicity

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MeSH Term

Cricetinae
Animals
Humans
Nipah Virus
Virulence
Henipavirus Infections
Mesocricetus
Genomics
Gene Expression Profiling
Journal Article
Article has an altmetric score of 7

Word Cloud

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