Polyethersulfone (PES) Filters Improve the Recovery of spp. and Enhance Selectivity against Interfering Microorganisms in Water Samples.
Pablo Casino, Asunción López, Sara Peiró, Santiago Rios, Aldous Porta, Gemma Agustí, Daniela Terlevich, Daniel Asensio, Ana María Marqués, Núria Piqué
Author Information
Pablo Casino: Department of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Josep Tura, 9H, Polígon Industrial Mas d'en Cisa, Sentmenat, 08181 Barcelona, Catalonia, Spain.
Asunción López: Department of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Josep Tura, 9H, Polígon Industrial Mas d'en Cisa, Sentmenat, 08181 Barcelona, Catalonia, Spain.
Sara Peiró: Department of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Josep Tura, 9H, Polígon Industrial Mas d'en Cisa, Sentmenat, 08181 Barcelona, Catalonia, Spain.
Santiago Rios: Department of Statistics, Biology Faculty, Universitat de Barcelona, Av. Diagonal, 643, 08028 Barcelona, Catalonia, Spain. ORCID
Aldous Porta: Department of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Josep Tura, 9H, Polígon Industrial Mas d'en Cisa, Sentmenat, 08181 Barcelona, Catalonia, Spain.
Gemma Agustí: Department of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Josep Tura, 9H, Polígon Industrial Mas d'en Cisa, Sentmenat, 08181 Barcelona, Catalonia, Spain.
Daniela Terlevich: Department of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Josep Tura, 9H, Polígon Industrial Mas d'en Cisa, Sentmenat, 08181 Barcelona, Catalonia, Spain.
Daniel Asensio: Department of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Josep Tura, 9H, Polígon Industrial Mas d'en Cisa, Sentmenat, 08181 Barcelona, Catalonia, Spain.
Ana María Marqués: Microbiology Section, Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona (UB), Av. Joan XXIII, 27-31, 08028 Barcelona, Catalonia, Spain. ORCID
Núria Piqué: Microbiology Section, Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona (UB), Av. Joan XXIII, 27-31, 08028 Barcelona, Catalonia, Spain. ORCID
In the analysis of Water samples, the type of filtration membrane material can influence the recovery of species, although this issue has been poorly investigated. Filtration membranes (0.45 µm) from different materials and manufacturers (numbered as 1, 2, 3, 4, and 5) were compared: mixed cellulose esters (MCEs), nitrocellulose (NC), and polyethersulfone (PES). After membrane filtration of samples, filters were placed directly onto GVPC agar and incubated at 36 ± 2 °C. The highest mean counts of colony-forming units and colony sizes for and were obtained with PES filters ( < 0.001). All membranes placed on GVPC agar totally inhibited and ATCC 19443 and ATCC 29212, whereas only the PES filter from manufacturer 3 (3-PES) totally inhibited . PES membrane performance also differed according to the manufacturer, with 3-PES providing the best productivity and selectivity. In real Water samples, 3-PES also produced a higher recovery and better inhibition of interfering microorganisms. These results support the use of PES membranes in methods where the filter is placed directly on the culture media and not only in procedures where membrane filtration is followed by a washing step (according to ISO 11731:2017).
