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Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Sep, 2023;35 (5): 484-491.

Assessing avian leukosis virus proviral load and lesion correlates in fowl glioma-inducing virus-infected Japanese bantam chickens.

Hayate Nishiura, Tomoe Nakajima, Shun Saito, Azusa Kato, Hitoshi Hatai, Kenji Ochiai
Author Information
  1. Hayate Nishiura: Laboratory of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan. ORCID
  2. Tomoe Nakajima: Laboratory of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
  3. Shun Saito: Laboratory of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
  4. Azusa Kato: Laboratory of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
  5. Hitoshi Hatai: Farm Animal Clinical Skills and Disease Control Center, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan. ORCID
  6. Kenji Ochiai: Laboratory of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
PMID: 37452573 DOI: 10.1177/10406387231186954

Abstract

The fowl glioma-inducing virus prototype (FGVp) and its variants, which belong to avian leukosis virus subgroup A (ALV-A), induce cardiomyocyte abnormalities and gliomas in chickens. However, the molecular mechanisms underlying these myocardial changes remain unclear, and ALV-induced tumorigenesis, which is caused by proviral insertional mutagenesis, does not explain the early development of cardiac changes in infected chickens. We established a quantitative PCR (qPCR) assay to measure ALV-A proviral loads in the brains and hearts of FGV-infected Japanese bantam chickens and compared these results with morphologic lesions. Four of 22 bantams had both gliomas and cardiac lesions. Hearts with cardiac lesions had a higher proviral load (10.3��������2.7 proviral copies/nucleus) than those without cardiac lesions (0.4��������0.4), suggesting that the proviral load in hearts is correlated with the frequency of myocardial changes. Our qPCR method may be useful in the study of ALV-induced cardiomyocyte abnormalities.

Keywords

avian leukosis virus cardiomyocyte fowl glioma-inducing virus glioma provirus qPCR

References

  1. Ann Neurol. 1991 Feb;29(2):194-201 [PMID: 2012389]
  2. PLoS One. 2014 Jan 23;9(1):e86546 [PMID: 24466146]
  3. Avian Pathol. 2002 Apr;31(2):193-9 [PMID: 12396365]
  4. Avian Pathol. 2003 Aug;32(4):383-90 [PMID: 17585462]
  5. J Vet Med Sci. 2008 May;70(5):469-74 [PMID: 18525168]
  6. J Vet Med Sci. 1991 Jun;53(3):399-408 [PMID: 1653036]
  7. Sci Rep. 2017 Oct 27;7(1):14271 [PMID: 29079779]
  8. Food Chem. 2017 Nov 1;234:472-478 [PMID: 28551263]
  9. Poult Sci. 2018 Nov 1;97(11):4048-4056 [PMID: 30184115]
  10. Sci Rep. 2020 May 22;10(1):8561 [PMID: 32444639]
  11. J Neurovirol. 1998 Dec;4(6):586-93 [PMID: 10065900]
  12. Front Physiol. 2021 May 14;12:676864 [PMID: 34054585]
  13. Rev Med Virol. 2008 Nov-Dec;18(6):387-405 [PMID: 18729235]
  14. Blood. 2012 Oct 25;120(17):3488-90 [PMID: 22955925]
  15. Proc Natl Acad Sci U S A. 2011 Jul 5;108(27):11199-204 [PMID: 21690402]
  16. Vet Pathol. 2006 May;43(3):294-301 [PMID: 16672576]
  17. J Acquir Immune Defic Syndr Hum Retrovirol. 1996;13 Suppl 1:S92-9 [PMID: 8797710]
  18. J Virol. 2004 Nov;78(21):11656-63 [PMID: 15479807]
  19. J Virol. 1988 Dec;62(12):4828-31 [PMID: 2846895]
  20. J Neurol Sci. 2005 Oct 15;237(1-2):53-9 [PMID: 15972218]
  21. Nucleic Acids Res. 2014;42(16):10209-25 [PMID: 25147212]
  22. Avian Pathol. 2003 Dec;32(6):617-24 [PMID: 14676013]
  23. AIDS Res Ther. 2017 Feb 16;14(1):9 [PMID: 28209198]
  24. Avian Pathol. 2012;41(3):299-309 [PMID: 22702458]
  25. Nucleic Acids Res. 2012 Aug;40(15):e115 [PMID: 22730293]
  26. Avian Pathol. 2020 Oct;49(5):440-447 [PMID: 32301629]
  27. J Virol. 2005 Sep;79(18):12035-44 [PMID: 16140779]
  28. J Vet Med Sci. 2002 May;64(5):395-9 [PMID: 12069070]
  29. Vet Rec. 1999 Jul 17;145(3):79-81 [PMID: 10460030]
  30. BMC Vet Res. 2019 Aug 13;15(1):288 [PMID: 31409367]
  31. Avian Pathol. 2008 Apr;37(2):127-37 [PMID: 18393089]
  32. Avian Pathol. 2011 Oct;40(5):499-505 [PMID: 21854177]
  33. Cell. 1981 Feb;23(2):323-34 [PMID: 6258798]
  34. AMB Express. 2020 Oct 7;10(1):181 [PMID: 33026522]
  35. J Gen Virol. 2004 Mar;85(Pt 3):647-652 [PMID: 14993650]
  36. J Natl Cancer Inst. 1968 Aug;41(2):373-8 [PMID: 5671285]
  37. J Gen Virol. 2002 Oct;83(Pt 10):2553-2561 [PMID: 12237439]
  38. Avian Dis. 2004 Dec;48(4):940-6 [PMID: 15666879]
  39. PLoS Biol. 2004 Aug;2(8):E234 [PMID: 15314653]
  40. Bioinformatics. 2007 May 15;23(10):1289-91 [PMID: 17379693]

MeSH Term

Animals
Avian Leukosis Virus
Chickens
Glioma
Poultry Diseases
Proviruses
Viral Load
Journal Article
Article has an altmetric score of 1

Word Cloud

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