Synthesis and Characterization of Zinc Oxide Nanoparticles at Different pH Values from L. and Their Assessment as an Antimicrobial Agent and Biomedical Application.
Hajira Arif, Sidra Qayyum, Wasim Akhtar, Iram Fatima, Waqas Khan Kayani, Khursheed Ur Rahman, Wedad A Al-Onazi, Amal M Al-Mohaimeed, Naila Khan Bangash, Nasra Ashraf, Sarah Abdul Razak, Asif Kamal, Sajid Ali
Author Information
Hajira Arif: Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad 13100, Pakistan.
Sidra Qayyum: Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad 13100, Pakistan.
Wasim Akhtar: Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad 13100, Pakistan.
Iram Fatima: Department of Biotechnology, Fatima Jinnah Women University, Rawalpindi 46000, Pakistan.
Waqas Khan Kayani: Department of Biotechnology, Faculty of Basic and Applied Sciences, University of Kotli, Azad Jammu and Kashmir, Kotli 11100, Pakistan. ORCID
Khursheed Ur Rahman: Department of Botany, Hazara University, Mansehra 21120, Pakistan.
Wedad A Al-Onazi: Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
Amal M Al-Mohaimeed: Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia. ORCID
Naila Khan Bangash: Environmental Microbiology Laboratory, Department of Agricultural Biological Chemistry, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
Nasra Ashraf: Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad 13100, Pakistan.
Sarah Abdul Razak: Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia. ORCID
Asif Kamal: Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Sajid Ali: Department of Horticulture and Life Science, Yeungnam University, Gyeongsan 38541, Republic of Korea. ORCID
The current study attempts to evaluate the formation, morphology, and physico-chemical properties of zinc oxide nanoparticles (ZnO NPs) synthesized from extract at different pH values and to investigate their antimicrobial and biomedical application potential. The reduction of zinc ions to ZnO NPs was determined by UV spectra, which revealed absorption peaks at 390 nm at pH 5 and 348 nm at pH 9, respectively. The spherical morphology of the nanoparticles was observed using scanning electron microscopy (SEM), and the size was 47 nm for pH 5 and 45 nm for pH 9. Fourier-transformed infrared spectroscopy (FTIR) was used to reveal the presence of functional groups on the surface of nanoparticles. The antibacterial activity was examined against , , and via the agar-well diffusion method. Comparatively, the highest activities were recorded at pH 9 against all bacterial strains, and among these, biogenic ZnO NPs displayed the maximum inhibition zone (i.e., 20.88 ± 0.79 mm) against . ZnO NPs prepared at pH 9 exhibited the highest antifungal activity of 80% at 25 mg/mL and antileishmanial activity of 82% at 400 mg/mL. Altogether, ZnO NPs synthesized at pH 9 show promising antimicrobial potential and could be used for biomedical applications.
