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BMC microbiology
08 02, 2023;23 (1): 208.

Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa.

Hadi Ghoomdost Noori, Omid Tadjrobehkar, Elham Moazamian
Author Information
  1. Hadi Ghoomdost Noori: Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
  2. Omid Tadjrobehkar: Department of Medical Microbiology (Bacteriology and Virology), Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran. o.tadjrobehkar@kmu.ac.ir.
  3. Elham Moazamian: Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
PMID: 37533040 DOI: 10.1186/s12866-023-02957-z

Abstract

BACKGROUND: Biofilm formation has reported as an important virulence associated properties of Pseudomonas aeruginosa that is regulated by quorum-sensing associated genes. Biofilm and quorum-sensing interfering properties of steroidal alkaloids, Solanidine and Solasodine were investigated in the present study.
RESULTS: Biofilm formation capacity and relative expression level of five studied genes(lasI, lasR, rhlI, rhlR and algD) were significantly increased dose-dependently after treatment with sub-inhibitory concentrations (32 and 512 µg/ml) of the both Solanidine and Solasodine. Biofilm formation capacity was more stimulated in weak biofilm formers(9 iaolates) in comparison to the strong biofilm producers(11 isolates). The lasI gene was the most induced QS-associated gene among five investigated genes.
CONCLUSION: Biofilm inducing properties of the plants alkaloids and probably medicines derived from them has to be considered for revision of therapeutic guidelines. Investigating the biofilm stimulating properties of corticosteroids and other medicines that comes from plant alkaloids also strongly proposed.

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MeSH Term

Pseudomonas aeruginosa
Biofilms
Quorum Sensing
Virulence Factors
Bacterial Proteins

Chemicals

solasodine
solanidine
Virulence Factors
Bacterial Proteins
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0BiofilmpropertiesformationgenesalkaloidsSolasodinebiofilmassociatedPseudomonasaeruginosaquorum-sensingSolanidineinvestigatedcapacityfivelasIgenemedicinesstimulatingBACKGROUND:reportedimportantvirulenceregulatedinterferingsteroidalpresentstudyRESULTS:relativeexpressionlevelstudiedlasRrhlIrhlRalgDsignificantlyincreaseddose-dependentlytreatmentsub-inhibitoryconcentrations32512 µg/mlstimulatedweakformers9iaolatescomparisonstrongproducers11isolatesinducedQS-associatedamongCONCLUSION:inducingplantsprobablyderivedconsideredrevisiontherapeuticguidelinesInvestigatingcorticosteroidscomesplantalsostronglyproposedactivitysolanidine

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