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PloS one
2023;18 (8): e0289556.

Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay.

Sofie Slott, Cecilie Schiøth Krüger-Jensen, Izabela Ferreira da Silva, Nadia Bom Pedersen, Kira Astakhova
Author Information
  1. Sofie Slott: Department of Chemistry, Technical University of Denmark, Kgs Lyngby, Denmark.
  2. Cecilie Schiøth Krüger-Jensen: Department of Chemistry, Technical University of Denmark, Kgs Lyngby, Denmark.
  3. Izabela Ferreira da Silva: Programa Interunidades de Pós-Graduacão em Bioinformática, Universidade Federal de Minas Gerais, Belo Horizonte-MG, Brazil.
  4. Nadia Bom Pedersen: Department of Chemistry, Technical University of Denmark, Kgs Lyngby, Denmark.
  5. Kira Astakhova: Department of Chemistry, Technical University of Denmark, Kgs Lyngby, Denmark. ORCID
PMID: 37607185 DOI: 10.1371/journal.pone.0289556

Abstract

We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had limit of detection of 2.2 pM. Thereby, the assay allowed identification of single-nucleotide polymorphism mismatch profiles in clinically relevant microRNA-128-2-3p, showing terminal mutations that correlate positively with inflammatory colitis and colorectal cancer.

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MeSH Term

Humans
Nucleic Acid Hybridization
Hybridization, Genetic
Mutation
Biological Assay
MicroRNAs

Chemicals

MicroRNAs
MIRN128 microRNA, human
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 8

Word Cloud

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