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08 22, 2023;13 (1): 13716.

Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells.

Juhi Pathak, Shiva Pratap Singh, Suresh Dinkar Kharche, Anjana Goel, Yogesh K Soni, Rakesh Kaushik, Megha Kose, Ashish Kumar
Author Information
  1. Juhi Pathak: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India.
  2. Shiva Pratap Singh: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India. shiva.singh@icar.gov.in.
  3. Suresh Dinkar Kharche: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India. kharche62@gmail.com.
  4. Anjana Goel: Department of Biotechnology, GLA University, Mathura, Uttar Pradesh, 281 406, India.
  5. Yogesh K Soni: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India.
  6. Rakesh Kaushik: Animal Genetics and Breeding Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India.
  7. Megha Kose: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India.
  8. Ashish Kumar: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh, 281 122, India.
PMID: 37607956 DOI: 10.1038/s41598-023-38634-4

Abstract

The enhanced availability of functional fibroblasts from precious tissue samples requires an ideal cell-culture system. Therefore, this study was designed to investigate the performance of caprine adult fibroblast cells (cadFibroblast) when cultivated in different culture media. The cadFibroblast cell lines from adult Barbari (Capra hircus) bucks were established and the effect of different media viz. DMEM/F-12 [with low-glucose (5.5 mM; DL) and high-glucose (30 mM; DH)], α-MEM [with low-glucose (5.5 mM; ML) and with high-glucose (30 mM; MH)], and fibroblast growth medium (FGM) were evaluated. Cells were then compared for growth characteristics and in-vitro dynamics through cellular morphology, proliferation, population-doubling time, double-immunocytochemistry, colony-forming units, wound healing, transwell migration, and differential expression of fibroblast-specific markers (FSP-1 and vimentin). The results of immunocytochemistry, transwell migration/invasion, and wound healing assays showed the superiority of DH over DL and other media tested. Whereas, similar effects of glucose supplementation and expression of FSP-1 were not observed in α-MEM. Transwell migration was significantly (p < 0.05) lower in FGM compared with other media tested. Overall, our results illustrate the media-dependent deviation in in-vitro dynamics and culture characteristics of cadFibroblasts that may be useful to develop strategies to cultivate these cells efficiently for research and downstream applications.

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MeSH Term

Cell Culture Techniques
Fibroblasts
Goats
Culture Media
In Vitro Techniques
Dermis
Animals
Cell Line
Male
Glucose
Gene Expression Profiling
Wound Healing
Cell Migration Assays
Biomarkers

Chemicals

Culture Media
Glucose
Biomarkers
Journal Article

Word Cloud

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