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Aug 09, 2023;11 (8):

Whole Genome Sequencing and Molecular Epidemiology of Clinical Isolates of from Algeria.

Rachida Namoune, Abla Djebbar, Rebecca Mekler, Martin McHugh, Mohammed El Amine Bekara, Arun Decano, Matthew T G Holden, Mohammed Sebaihia
Author Information
  1. Rachida Namoune: Laboratory of Molecular Biology, Genomics and Bioinformatics, Department of Biology, Faculty of Nature and Life Sciences, University Hassiba Benbouali of Chlef, Chlef 02000, Algeria. ORCID
  2. Abla Djebbar: Laboratory of Molecular Biology, Genomics and Bioinformatics, Department of Biology, Faculty of Nature and Life Sciences, University Hassiba Benbouali of Chlef, Chlef 02000, Algeria.
  3. Rebecca Mekler: School of Medicine, University of St Andrews, St Andrews KY16 9TF, UK.
  4. Martin McHugh: School of Medicine, University of St Andrews, St Andrews KY16 9TF, UK.
  5. Mohammed El Amine Bekara: Laboratory of Molecular Biology, Genomics and Bioinformatics, Department of Biology, Faculty of Nature and Life Sciences, University Hassiba Benbouali of Chlef, Chlef 02000, Algeria.
  6. Arun Decano: School of Medicine, University of St Andrews, St Andrews KY16 9TF, UK.
  7. Matthew T G Holden: School of Medicine, University of St Andrews, St Andrews KY16 9TF, UK.
  8. Mohammed Sebaihia: Laboratory of Molecular Biology, Genomics and Bioinformatics, Department of Biology, Faculty of Nature and Life Sciences, University Hassiba Benbouali of Chlef, Chlef 02000, Algeria.
PMID: 37630607 DOI: 10.3390/microorganisms11082047

Abstract

is an important pathogen responsible for various healthcare- and community-acquired infections. In this study, whole genome sequencing (WGS) was used to genotype clinical isolates from two hospitals in Algeria and to characterize their genetic determinants of antimicrobial resistance. Seventeen isolates were included in this study. WGS, single-nucleotide polymorphism (SNP)-based phylogenetic analysis, in silico multilocus sequence typing (MLST), and staphylococcal cassette chromosome (SCC) typing and in silico antimicrobial resistance profiling were performed. Phenotypic antibiotic susceptibility testing was performed using the Vitek 2 system and the disk diffusion method. The isolates were separated into sequence types (STs), with ST80 being predominant; five clonal complexes (CCs); four types (t044, t127, t368, t386); and two SCC types (IVc and IVa). Whole genome analysis revealed the presence of the resistance genes , , , , , , (3')-IIIa and (6) and mutations conferring resistance in the genes and . The rate of multidrug resistance (MDR) was 64%. This work provides a high-resolution characterization of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) isolates and emphasizes the importance of continuous surveillance to monitor the spread of in healthcare settings in the country.

Keywords

Algeria MLST MRSA Staphylococcus aureus WGS antibiotic resistance

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Grants

  1. MR/S004785/1/Medical Research Council
Journal Article
Article has an altmetric score of 2

Word Cloud

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