Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor.
Armando G Salinas, Jeong Oen Lee, Shana M Augustin, Shiliang Zhang, Tommaso Patriarchi, Lin Tian, Marisela Morales, Yolanda Mateo, David M Lovinger
Author Information
Armando G Salinas: Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA. armando.salinas@lsuhs.edu. ORCID
Jeong Oen Lee: Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA.
Shana M Augustin: Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA.
Shiliang Zhang: Confocal and Electron Microscopy Core, National Institute on Drug Abuse, Baltimore, MD, USA. ORCID
Tommaso Patriarchi: Department of Biochemistry and Molecular Medicine, University of California at Davis, Davis, CA, USA. ORCID
Lin Tian: Department of Biochemistry and Molecular Medicine, University of California at Davis, Davis, CA, USA. ORCID
Marisela Morales: Neuronal Networks Section, Integrative Neuroscience Research Branch, National Institute on Drug Abuse, Baltimore, MD, USA. ORCID
Yolanda Mateo: Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA.
David M Lovinger: Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA. lovindav@mail.nih.gov. ORCID
The development of genetically encoded dopamine sensors such as dLight has provided a new approach to measuring slow and fast dopamine dynamics both in brain slices and in vivo, possibly enabling dopamine measurements in areas like the dorsolateral striatum (DLS) where previously such recordings with fast-scan cyclic voltammetry (FSCV) were difficult. To test this, we first evaluated dLight photometry in mouse brain slices with simultaneous FSCV and found that both techniques yielded comparable results, but notable differences in responses to dopamine transporter inhibitors, including cocaine. We then used in vivo fiber photometry with dLight in mice to examine responses to cocaine in DLS. We also compared dopamine responses during Pavlovian conditioning across the striatum. We show that dopamine increases were readily detectable in DLS and describe transient dopamine kinetics, as well as slowly developing signals during conditioning. Overall, our findings indicate that dLight photometry is well suited to measuring dopamine dynamics in DLS.
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