Self-Reporting Theranostic: Nano Tool for Arterial Thrombosis.
Suryyani Deb, Mohammad Azharuddin, Sofia Ramström, Kanjaksha Ghosh, Santiswarup Singha, Thobias Romu, Hirak Kumar Patra
Author Information
Suryyani Deb: Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Haringhata 741249, India.
Mohammad Azharuddin: Cambridge Display Technology Ltd., Huntingdon PE29 2XG, UK.
Sofia Ramström: School of Medical Sciences, Orebro University, 701 82 Örebro, Sweden. ORCID
Kanjaksha Ghosh: National Institute of Immunohaematology, KEM Hospital Campus, Mumbai 400012, India. ORCID
Santiswarup Singha: National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India.
Thobias Romu: Centre for Medical Image Science and Visualization (CMIV), Department of Biomedical Engineering (IMT), Linköping University, 581 85 Linköping, Sweden. ORCID
Hirak Kumar Patra: Department of Surgical Biotechnology, UCL Division of Surgery and Interventional Sciences, University College London, London NW3 2PF, UK. ORCID
Arterial thrombosis (AT) originates through platelet-mediated thrombus formation in the blood vessel and can lead to heart attack, stroke, and peripheral vascular diseases. Restricting the thrombus growth and its simultaneous monitoring by visualisation is an unmet clinical need for a better AT prognosis. As a proof-of-concept, we have engineered a nanoparticle-based theranostic (combined therapy and monitoring) platform that has the potential to monitor and restrain the growth of a thrombus concurrently. The theranostic nanotool is fabricated using biocompatible super-paramagnetic iron oxide nanoparticles (SPIONs) as a core module tethered with the anti-platelet agent Abciximab (ReoPro) on its surface. Our in vitro feasibility results indicate that ReoPro-conjugated SPIONS (Tx@ReoPro) can effectively prevent thrombus growth by inhibiting fibrinogen receptors (GPIIbIIIa) on the platelet surface, and simultaneously, it can also be visible through non-invasive magnetic resonance imaging (MRI) for potential reporting of the real-time thrombus status.
