Investigating the Significance of Non-/ Strains in Patients with Diarrhea.

Nermin Teksoy, Mehmet Ilktac, Betigul Ongen
Author Information
  1. Nermin Teksoy: Medical Microbiology Department, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey. ORCID
  2. Mehmet Ilktac: Faculty of Pharmacy, Eastern Mediterranean University, via Mersin 10 Turkey, Famagusta 99628, Cyprus. ORCID
  3. Betigul Ongen: Medical Microbiology Department, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey. ORCID

Abstract

is one of the most commonly reported foodborne bacteria worldwide. Although and have been reported to be responsible for the great majority of campylobacteriosis, the burden of infections by species other than and have been increasing as a result of a transition to diagnostic test methods that enable the isolation of emerging species. The aim of the present study was to recover , , and emerging species from the stool samples of 500 patients with gastroenteritis and 100 healthy subjects via the use of a filtration method and culture techniques using Butzler agar and mCCDA under a microaerobic or hydrogen-enriched atmosphere, identify the species by multiplex PCR methods and assess the significance of emerging species in enteric diseases. Thirty-one (6.2%) spp. were isolated from the stool samples of diarrheic patients but none from healthy individuals. Of 31 isolates, 21 (67.8%), nine (29%), and one (3.2%) were identified as , , and by multiplex PCR, respectively. The filtration method was superior to the culture technique using mCCDA under a microaerobic atmosphere. was evaluated as the etiology of gastroenteritis as a result of laboratory and clinical evaluations. The present study was the first to indicate that emerging species are rarely detected and is linked to acute gastroenteritis in Turkey where additional studies are warranted to clarify the significance of emerging species in gastroenteritis.

Keywords

References

  1. J Clin Microbiol. 2017 Jul;55(7):2204-2207 [PMID: 28468859]
  2. MMWR Morb Mortal Wkly Rep. 2019 Apr 26;68(16):369-373 [PMID: 31022166]
  3. EFSA J. 2023 Mar 06;21(3):e07867 [PMID: 36891283]
  4. Epidemiol Infect. 2012 Apr;140(4):684-8 [PMID: 21676357]
  5. Clin Microbiol Rev. 2019 Jul 3;32(4): [PMID: 31270126]
  6. Emerg Microbes Infect. 2018 Apr 11;7(1):64 [PMID: 29636463]
  7. Mikrobiyol Bul. 2013 Apr;47(2):230-9 [PMID: 23621723]
  8. Appl Microbiol Biotechnol. 2020 Dec;104(24):10409-10436 [PMID: 33185702]
  9. J Clin Microbiol. 1996 Sep;34(9):2304-6 [PMID: 8862606]
  10. Clin Lab. 2020 Mar 1;66(3): [PMID: 32162883]
  11. Jpn J Infect Dis. 2017 Jul 24;70(4):455-457 [PMID: 28250261]
  12. Clin Microbiol Infect. 2013 May;19(5):445-50 [PMID: 22512739]
  13. Scand J Infect Dis. 1995;27(2):187-8 [PMID: 7660089]
  14. MMWR Morb Mortal Wkly Rep. 2021 Sep 24;70(38):1332-1336 [PMID: 34555002]
  15. Emerg Infect Dis. 2012 Mar;18(3):510-2 [PMID: 22377283]
  16. Int J Environ Res Public Health. 2019 Jun 21;16(12): [PMID: 31234440]
  17. J Med Microbiol. 2019 Feb;68(2):136-142 [PMID: 30540246]
  18. J Med Microbiol. 2016 Mar;65(3):219-226 [PMID: 26698172]
  19. J Clin Microbiol. 1997 Oct;35(10):2568-72 [PMID: 9316909]
  20. Diagn Microbiol Infect Dis. 2015 Sep;83(1):11-2 [PMID: 26022656]
  21. Res Microbiol. 1996 Nov-Dec;147(9):707-18 [PMID: 9296105]
  22. Mol Cell Probes. 1995 Aug;9(4):247-50 [PMID: 7477020]
  23. J Clin Microbiol. 2000 Jul;38(7):2798-9 [PMID: 10979752]
  24. PLoS One. 2021 Jan 27;16(1):e0245951 [PMID: 33503068]
  25. APMIS. 2020 Jan;128(1):41-47 [PMID: 31692136]
  26. EFSA J. 2022 Dec 13;20(12):e07666 [PMID: 36524203]
  27. Front Cell Infect Microbiol. 2012 Feb 03;2:4 [PMID: 22919596]
  28. Pediatr Infect Dis J. 2018 Dec;37(12):e339-e341 [PMID: 29601450]
  29. J Clin Microbiol. 2011 Jan;49(1):209-19 [PMID: 21106791]
  30. Int J Food Microbiol. 2011 Nov 1;150(2-3):171-7 [PMID: 21855156]
  31. Front Cell Infect Microbiol. 2018 Jul 24;8:243 [PMID: 30087857]
  32. Diagn Microbiol Infect Dis. 2013 Apr;75(4):434-6 [PMID: 23375869]
  33. Clin Microbiol Rev. 2015 Jul;28(3):687-720 [PMID: 26062576]
  34. Front Cell Infect Microbiol. 2022 Nov 28;12:979055 [PMID: 36519137]
  35. Pathogens. 2022 Jan 24;11(2): [PMID: 35215086]
  36. Emerg Infect Dis. 2004 Oct;10(10):1863-7 [PMID: 15504280]
  37. J Clin Microbiol. 2004 Dec;42(12):5549-57 [PMID: 15583280]

Grants

  1. 58247/Istanbul University Scientific Research Foundation

Word Cloud

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