Huan-Ju Wang: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Ce Cui: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Cheng-Xi Li: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Hao Guo: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Ya-Ling Wang: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Yu-Dan Huang: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Jian-Lin Jiang: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Xue-Mei Luo: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Shuai Zhao: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Jia-Xun Feng: State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Due to the scarcity of wild fruiting bodies, submerged fermentation of the medicinal fungus is attracting much attention, but the production of bioactive triterpenoids is low. Therefore, there is an urgent need to improve the triterpenoid yield of submerged fermentation. Here, the mutant E3-64 was generated from strain AC16101 through random mutagenesis breeding, producing 172.8 mg triterpenoid per gram of dry mycelia. Further optimization of culture parameters resulted in a yield of 255.5 mg/g dry mycelia (i.e., an additional >1.4-fold increase), which is the highest reported yield thus far. Notably, mutant E3-64 produced 94% and 178% more of the triterpenoid components antcin A and antcamphin A, respectively, while it produced 52% and 15% less antcin B and G, respectively. Mutant E3-64 showed increased expression of key genes involved in triterpenoid biosynthesis, as well as different genome-wide single-nucleotide polymorphisms as compared with AC16101. Triterpenoids of the E3-64 mycelia exhibited remarkably protective activity against acute CCl-induced liver injury in mice. This study shows the potential of for scientific research and commercial application.
