Essential Oil from Has Antiquorum Sensing and Antibiofilm Potential against Strain PAO1.
Palwasha Khan, Amara Waheed, Muhammad Azeem, Amna Parveen, Muhammad Arfat Yameen, Jamshed Iqbal, Muhammad Ali, Shiwei Wang, Sadaf Qayyum, Awal Noor, Tatheer Alam Naqvi
Author Information
Palwasha Khan: Department of Biotechnology, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22044, Pakistan.
Amara Waheed: Department of Biotechnology, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22044, Pakistan.
Muhammad Azeem: Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22044, Pakistan.
Amna Parveen: Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22044, Pakistan.
Muhammad Arfat Yameen: Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22044, Pakistan.
Jamshed Iqbal: Centre for Advanced Drug Research, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22044, Pakistan.
Muhammad Ali: Department of Biotechnology, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22044, Pakistan.
Shiwei Wang: Key Laboratory of Resources Biology and Biotechnology in Western China, School of Life Sciences, Northwest University, Ministry of Education, Xi'an 710069, China.
Sadaf Qayyum: Department of Basic Sciences, Preparatory Year Deanship, King Faisal University, Al-Hofuf 31982, Al-Hassa, Saudi Arabia.
Awal Noor: Department of Basic Sciences, Preparatory Year Deanship, King Faisal University, Al-Hofuf 31982, Al-Hassa, Saudi Arabia.
Tatheer Alam Naqvi: Department of Biotechnology, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22044, Pakistan. ORCID
Biofilms are complex communities of microorganisms that are enclosed in a matrix that shows increased resistance to antimicrobial and immunological encounters. Mostly, the traditional methods to control biofilm are exhausted; therefore, the aim is to evaluate the potential of essential oil (EO) from to encounter biofilm and other related virulence factors. The EO of was extracted through steam-distillation, analyzed on gas chromatography-mass spectrometry, and the biofilm inhibition assays were performed with various concentrations of EO. Mainly the EO from contains -β-ocimene (29.1%), -tagetenone (23.1%), and -tagetenone (17.7%). The virulence factors were monitored while applying different concentrations of EO and it was recorded that the EO from significantly inhibited the virulence factors linked with quorum sensing (QS), such as pyocyanin production, protease production, and swarming motility. Biofilm formation is one of the most important virulence factors associated with the QS pathway and was inhibited up to 79% in the presence of EO. Antibacterial activity against the PAO1 of EO was not so promising particularly and it has high MIC (325 μg/mL) and MBC (5000 μg/mL). EO is quite efficient to inhibit biofilm in a very small concentration of 20 μg/mL, which confirms that the biofilm inhibition by EO is not by killing bacterial cells but by inhibiting the QS pathway. The study on PAO1 constructs carrying various QS reported genes confirmed that the EO interferes with the QS pathway that ultimately controls various virulence factors caused by PAO1.
