Oreoch-1: A Peptide from as a Potential Tool against Staphylococci.
Francesca Palma, Annalisa Chianese, Erica Panico, Giuseppe Greco, Alessandra Fusco, Vittoria Savio, Eleonora Ruocco, Alessandra Monti, Nunzianna Doti, Carla Zannella, Giovanna Donnarumma, Anna De Filippis, Massimiliano Galdiero
Author Information
Francesca Palma: Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Annalisa Chianese: Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy. ORCID
Erica Panico: UOC of Virology and Microbiology, University Hospital of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Giuseppe Greco: UOC of Virology and Microbiology, University Hospital of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Alessandra Fusco: Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy. ORCID
Vittoria Savio: Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Eleonora Ruocco: Dermatology Unit, Department of Mental and Physical Health and Preventive Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Alessandra Monti: Institute of Biostructures and Bioimaging (IBB), National Research Council (CNR), 80131 Naples, Italy.
Nunzianna Doti: Institute of Biostructures and Bioimaging (IBB), National Research Council (CNR), 80131 Naples, Italy. ORCID
Carla Zannella: Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy. ORCID
Giovanna Donnarumma: Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy. ORCID
Anna De Filippis: Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy. ORCID
Massimiliano Galdiero: Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy. ORCID
Staphylococci, including and , are important human pathogens associated with potentially life-threatening infections. Their great biofilm-producing ability and the development of resistance mechanisms often account for therapeutic failure. Hence, the scientific community has devoted intensive efforts to the development of antimicrobial compounds active against both planktonic and sessile bacterial populations. Contextually, antimicrobial peptides (AMPs) are natural peptides produced by the innate immunity of every organism, representing a potential new therapeutic solution against human microbial pathogens. Our work focused on the in vitro activity of Oreoch-1, an AMP from the gills of Nile tilapia (), against standard and clinical and strains. Firstly, the cytotoxicity profile of Oreoch-1 was determined in human colon carcinoma cells. Secondly, its antibacterial spectrum was explored against staphylococcal strains to set up the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Our results highlighted an antibacterial activity in the range 6.25-25 μM, with a general bacteriostatic effect. Therefore, the biofilm-inhibitory property was assessed against ATCC 25923 and ATCC 35984, indicating a significant reduction in biomass at sub-MIC concentrations. Overall, our study indicates Oreoch-1 as a promising new therapeutic weapon against staphylococcal infections.
