The degradation of green leaves in autumn after their photosynthetic activities is associated with decreases in chlorophylls and increases in anthocyanins. However, the sequential orders of these processes are not well understood because of a lack of continuous monitoring of leaves in the same positions. Therefore, the senescence processes of Japanese maple () leaves were followed daily in the same positions for approximately 60 days using visible spectroscopy with an original handheld visible-near-infrared spectrometer. The obtained reflection spectra were converted to absorption spectra and band areas of chlorophyll a and anthocyanins were determined. Decreases in the chlorophyll a band area with time show two-step exponential decreases corresponding to slow and fast first-order decrease rates. A rapid decrease in chlorophyll a started after an increase in anthocyanin. Therefore, the leaf senescence started through a slow decrease in chlorophyll a (20-30 days), followed by a rapid increase in anthocyanins (~20 days), followed by a rapid decrease in chlorophyll a (10-20 days). The formation of anthocyanins has been proposed to protect leaf cells from losing chlorophylls through solar radiation damage. The obtained sequential changes of pigments support this light screen hypothesis. (199 words < 200 words).
