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Archives of microbiology
Oct 31, 2023;205 (12): 365.

Evaluation of glycyl-arginine and lysyl-aspartic acid dipeptides for their antimicrobial, antibiofilm, and anticancer potentials.

Handan Sevim Akan, Gülcan Şahal, Tuğçe Deniz Karaca, Özer Aylin Gürpınar, Meltem Maraş, Alev Doğan
Author Information
  1. Handan Sevim Akan: Department Biology, Faculty of Science, Hacettepe University, Beytepe, Cankaya, 06800, Ankara, Turkey. sevimh@hacettepe.edu.tr. ORCID
  2. Gülcan Şahal: Department Biology, Faculty of Science, Hacettepe University, Beytepe, Cankaya, 06800, Ankara, Turkey. ORCID
  3. Tuğçe Deniz Karaca: Department of Medical Services and Techniques, Gazi University Health Service Vocational School, Ankara, Turkey. ORCID
  4. Özer Aylin Gürpınar: Department Biology, Faculty of Science, Hacettepe University, Beytepe, Cankaya, 06800, Ankara, Turkey. ORCID
  5. Meltem Maraş: Department of Mathematics and Science Education, Faculty of Education Ereğli, Bülent Ecevit University, Zonguldak, Turkey. ORCID
  6. Alev Doğan: Department of Science Education, Faculty of Gazi Education, Gazi University, Teknikokullar, Ankara, Turkey. ORCID
PMID: 37906313 DOI: 10.1007/s00203-023-03724-4

Abstract

Antibacterial resistance and cancer are worldwide challenges and have been defined as major threats by international health organizations. Peptides are produced naturally by all organisms and have a variety of immunomodulatory, physiological, and wound-healing properties. They can also provide protection against microorganisms and tumor cells. Therefore, we aimed to determine the antimicrobial, antibiofilm, and anticancer potentials of glycyl-arginine and Lysyl-Aspartic acid dipeptides. The Broth Dilution and Crystal Violet Binding assays assessed the antimicrobial tests and biofilm inhibitory effects. The MTT assay was used to measure the cytotoxic effects of dipeptides on HeLa cell viability. According to our results, Candida tropicalis T26 and Proteus mirabilis U15 strains were determined as more resistant to Staphylococcus epidermidis W17 against glycyl-arginine and Lysyl-Aspartic acid dipeptides with MICs higher than 2 mM (1 mg/mL). Sub-MICs of glycyl-arginine caused inhibitions against biofilm formation of all the tested clinical isolates, with the highest inhibition observed against S. epidermidisW17. Lysyl-Aspartic acid exhibited zero to no effect against biofilm formation of P. mirabilisU15, and S. epidermidisW17, whereas it exhibited 52% inhibition of biofilm formation of C. tropicalisT26. Cell viability results revealed that HeLa cell viability decreases with increasing concentration of both dipeptides. Also, parallel to antimicrobial tests, glycyl-arginine has a greater cytotoxic effect compared to Lysyl-Aspartic acid. The findings from this study will contribute to the advancement of novel strategies involving dipeptide-based synthesizable molecules and drug development studies. However, it is essential to note that there are still challenges, including the need for extensive experimental and clinical trials.

Keywords

Antibiofilm effect Anticancer activity Antimicrobials Cytotoxicity Glycyl-arginine Lysyl-aspartic acid

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MeSH Term

Humans
Anti-Bacterial Agents
Anti-Infective Agents
Aspartic Acid
Biofilms
Dipeptides
HeLa Cells
Microbial Sensitivity Tests
Antineoplastic Agents

Chemicals

Anti-Bacterial Agents
Anti-Infective Agents
Aspartic Acid
Dipeptides
Antineoplastic Agents
Journal Article
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Word Cloud

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