The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens.
Diana R Baidamshina, Elena Yu Trizna, Svetlana S Goncharova, Andrey V Sorokin, Maria S Lavlinskaya, Anastasia P Melnik, Leysan F Gafarova, Maya A Kharitonova, Olga V Ostolopovskaya, Valeriy G Artyukhov, Evgenia A Sokolova, Marina G Holyavka, Mikhail I Bogachev, Airat R Kayumov, Pavel V Zelenikhin
Author Information
Diana R Baidamshina: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia.
Elena Yu Trizna: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia. ORCID
Svetlana S Goncharova: Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia.
Andrey V Sorokin: Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia. ORCID
Maria S Lavlinskaya: Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia. ORCID
Anastasia P Melnik: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia. ORCID
Leysan F Gafarova: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia.
Maya A Kharitonova: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia. ORCID
Olga V Ostolopovskaya: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia.
Valeriy G Artyukhov: Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia.
Evgenia A Sokolova: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia. ORCID
Marina G Holyavka: Department of Biophysics and Biotechnology, Voronezh State University, 394018 Voronezh, Russia. ORCID
Mikhail I Bogachev: Biomedical Engineering Research Centre, St. Petersburg Electrotechnical University, 197022 St. Petersburg, Russia. ORCID
Airat R Kayumov: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia. ORCID
Pavel V Zelenikhin: Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia. ORCID
In the last decade, Ficin, a proteolytic enzyme extracted from the latex sap of the wild fig tree, has been widely investigated as a promising tool for the treatment of microbial biofilms, wound healing, and oral care. Here we report the antibiofilm properties of the enzyme immobilized on soluble carboxymethyl chitosan (CMCh) and CMCh itself. Ficin was immobilized on CMCh with molecular weights of either 200, 350 or 600 kDa. Among them, the carrier with a molecular weight of 200 kDa bound the maximum amount of enzyme, binding up to 49% of the total protein compared to 19-32% of the total protein bound to other CMChs. Treatment with pure CMCh led to the destruction of biofilms formed by , , , and , while no apparent effect on was observed. A soluble Ficin was less efficient in the destruction of the biofilms formed by and . By contrast, treatment with CMCh200-immobilized Ficin led to a significant reduction of the biofilms of the primary colonizers and In model biofilms obtained by the inoculation of swabs from teeth of healthy volunteers, the destruction of the biofilm by both soluble and immobilized Ficin was observed, although the degree of the destruction varied between artificial plaque samples. Nevertheless, combined treatment of oral biofilm by enzyme and chlorhexidine for 3 h led to a significant decrease in the viability of biofilm-embedded cells, compared to solely chlorhexidine application. This suggests that the use of either soluble or immobilized Ficin would allow decreasing the amount and/or concentration of the antiseptics required for oral care or improving the efficiency of oral cavity sanitization.
