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Nov 10, 2023;11 (11):

Serological Evidence of Hepatitis E Virus Infection in Brazilian Equines.

Caroline Roberta Soares Salgado, Aldaleia do Nascimento E Silva, Igor Falco Arruda, Patrícia Riddell Millar, Maria Regina Reis Amendoeira, Luciane Almeida Amado Leon, Raffaella Bertoni Cavalcanti Teixeira, Jorge Tiburcio Barbosa de Lima, Flávia Löwen Levy Chalhoub, Ana Maria Bispo de Filippis, Ana Beatriz Monteiro Fonseca, Jaqueline Mendes de Oliveira, Marcelo Alves Pinto, Andreza Soriano Figueiredo
Author Information
  1. Caroline Roberta Soares Salgado: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil.
  2. Aldaleia do Nascimento E Silva: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil.
  3. Igor Falco Arruda: Laboratório de Toxoplasmose e outras Protozooses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil.
  4. Patrícia Riddell Millar: Laboratório de Toxoplasmose e outras Protozooses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil.
  5. Maria Regina Reis Amendoeira: Laboratório de Toxoplasmose e outras Protozooses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil. ORCID
  6. Luciane Almeida Amado Leon: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil.
  7. Raffaella Bertoni Cavalcanti Teixeira: Clínica de Grandes Animais, Departamento de Medicina Veterinária, Universidade Federal de Viçosa-UFV, Viçosa 36570-900, MG, Brazil. ORCID
  8. Jorge Tiburcio Barbosa de Lima: Departamento de Clínica e Cirurgia Veterinárias, Universidade Federal de Minas Gerais-UFMG, Belo Horizonte 31270-901, MG, Brazil. ORCID
  9. Flávia Löwen Levy Chalhoub: Laboratório de Arbovírus e Vírus Hemorrágicos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil.
  10. Ana Maria Bispo de Filippis: Laboratório de Arbovírus e Vírus Hemorrágicos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil.
  11. Ana Beatriz Monteiro Fonseca: Departamento de Estatística, Instituto de Matemática e Estatística, Universidade Federal Fluminense-UFF, Niterói 24210-346, RJ, Brazil.
  12. Jaqueline Mendes de Oliveira: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil. ORCID
  13. Marcelo Alves Pinto: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil. ORCID
  14. Andreza Soriano Figueiredo: Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil. ORCID
PMID: 38004754 DOI: 10.3390/microorganisms11112743

Abstract

Hepatitis E virus (HEV) infection has been demonstrated in various animal species; those recognized as potential zoonotic reservoirs pose a considerable risk to public health. In Brazil, HEV-3 is the only genotype identified in humans and swine nationwide, in a colony-breeding cynomolgus monkey and, recently, in bovines and capybara. There is no information regarding HEV exposure in the equine population in Brazil. This study aimed to investigate anti-HEV antibodies and viral RNA in serum samples from horses slaughtered for meat export and those bred for sport/reproduction purposes. We used a commercially available ELISA kit modified to detect species-specific anti-HEV, using an anti-horse IgG-peroxidase conjugate and evaluating different cutoff formulas and assay precision. Serum samples (n = 257) were tested for anti-HEV IgG and HEV RNA by nested RT-PCR and RT-qPCR. The overall anti-HEV seroprevalence was 26.5% (68/257) without the detection of HEV RNA. Most municipalities (53.3%) and farms (58.8%) had positive horses. Animals slaughtered for human consumption had higher risk of HEV exposure (45.5%) than those bred for sports or reproduction (6.4%) ( < 0.0001). The statistical analysis revealed sex and breeding system as possible risk-associated factors. The first serological evidence of HEV circulation in Brazilian equines reinforces the need for the surveillance of HEV host expansion in a one-health approach.

Keywords

equine anti-HEV IgG hepatitis E virus horses zoonosis

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Grants

  1. no grant number/Oswaldo Cruz Foundation
Journal Article
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Word Cloud

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