Background This study aimed to environmentally synthesize zinc oxide nanoparticles (ZnO-NPs) using (AC) extract and evaluated their antimicrobial efficacy against , , and . Methodology AC extract was combined with a zinc sulfate solution to synthesize ZnO-NPs. The NPs were characterized using UV-visible spectroscopy, Fourier transform infrared (FTIR) analysis, scanning electron microscopy (SEM), and energy-dispersive electron microscopy (EDX). Antimicrobial activity was assessed using the agar disc diffusion method against , , and . Results Green synthesis of ZnO-NPs with AC extract yielded NPs of different sizes and shapes. SEM analysis showed circular and conical NPs measuring up to 10 nm. EDX analysis confirmed the presence of zinc (Zn) and oxygen (O) particles. UV-visible spectroscopy indicated ZnO-NP formation with a peak at 290 nm. These NPs exhibited strong antimicrobial activity against , with larger inhibition zones at higher concentrations, i.e., 15 mm at 100 μL. Whereas they showed low activity of 12 mm at 100 μL against and showed no activity against . Conclusions Environmentally friendly synthesis of ZnO-NPs using AC extract provides an effective method for NP production. It exhibits strong antimicrobial activity against , indicating the potential for targeted antimicrobial solutions in addressing associated infections.
