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Jan 11, 2024;12 (1): e0251023.

Overexpression of efflux pump genes is one of the mechanisms causing drug resistance in .

Ying Long, Bin Wang, Tiancheng Xie, Ruixin Luo, Jing Tang, Jianping Deng, Chuan Wang
Author Information
  1. Ying Long: West China School of Public Health and West China Fourth Hospital, Sichuan University , Chengdu, China.
  2. Bin Wang: Zigong Center for Disease Control and Prevention , Zigong, China.
  3. Tiancheng Xie: West China School of Public Health and West China Fourth Hospital, Sichuan University , Chengdu, China. ORCID
  4. Ruixin Luo: Zigong Center for Disease Control and Prevention , Zigong, China.
  5. Jing Tang: West China School of Public Health and West China Fourth Hospital, Sichuan University , Chengdu, China.
  6. Jianping Deng: Zigong Center for Disease Control and Prevention , Zigong, China. ORCID
  7. Chuan Wang: West China School of Public Health and West China Fourth Hospital, Sichuan University , Chengdu, China. ORCID
PMID: 38047702 DOI: 10.1128/spectrum.02510-23

Abstract

IMPORTANCE: Gene mutations cannot explain all drug resistance of , and the overexpression of efflux pump genes is considered another important cause of drug resistance. A total of 46 clinical isolates were included in this study to analyze the overexpression of efflux pump genes in different resistant types of strains. The results showed that overexpression of efflux pump genes did not occur in sensitive strains. There was no significant trend in the overexpression of efflux pump genes before and after one-half of MIC drug induction. By adding the efflux pump inhibitor verapamil, we can observe the decrease of MIC of some drug-resistant strains. At the same time, this study ensured the reliability of calculating the relative expression level of efflux pump genes by screening reference genes and using two reference genes for the normalization of quantitative PCR. Therefore, this study confirms that the overexpression of efflux pump genes plays an important role in the drug resistance of clinical isolates of .

Keywords

MIRU-VNTR Mycobacterium tuberculosis drug resistance mechanism efflux pump gene mutation verapamil

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Grants

  1. No. 2022-YF09-00011-SN/Major Scientific and Technological Application Demonstration Project of Chengdu Science and Technology Bureau
  2. No. 2017HH0080/International Cooperation Project of Sichuan Province Science and Technology Department
  3. No. 2020YLSF13/Key Science and Technology Planning Project of Zigong

MeSH Term

Mycobacterium tuberculosis
Antitubercular Agents
Reproducibility of Results
Bacterial Proteins
Microbial Sensitivity Tests
Drug Resistance

Chemicals

Antitubercular Agents
Bacterial Proteins
Journal Article
Article has an altmetric score of 1

Word Cloud

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