Isolation of temperate bacteriophage with broad killing activity to clinical isolates.
Katsuhito Sugai, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Yo Sugawara, Junzo Hisatsune, Jumpei Fujiki, Hidetomo Iwano, Kotaro Tanimoto, Motoyuki Sugai, Hitoshi Komatsuzawa
Author Information
Katsuhito Sugai: Department of Orthodontics, Hiroshima University Graduate School of Biomedical and Health Sciences, 1-2-3, Kasumi,Minamiku, Hiroshima-shi, Hiroshima 734-8553, Japan.
Miki Kawada-Matsuo: Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, 1-2-3,Kasumi,Minamiku, Hiroshima-shi, Hiroshima 734-8553, Japan.
Mi Nguyen-Tra Le: Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, 1-2-3,Kasumi,Minamiku, Hiroshima-shi, Hiroshima 734-8553, Japan.
Yo Sugawara: Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjukuku, Tokyoto, Japan.
Junzo Hisatsune: Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjukuku, Tokyoto, Japan.
Jumpei Fujiki: Laboratory of Veterinary Biochemistry, Department of Veterinary Medicine, Rakuno Gakuen University, 568,Bunkyodaimidorimachi, Ebetsu-shi, Hokkaido 069-0836, Japan.
Hidetomo Iwano: Laboratory of Veterinary Biochemistry, Department of Veterinary Medicine, Rakuno Gakuen University, 568,Bunkyodaimidorimachi, Ebetsu-shi, Hokkaido 069-0836, Japan.
Kotaro Tanimoto: Department of Orthodontics, Hiroshima University Graduate School of Biomedical and Health Sciences, 1-2-3, Kasumi,Minamiku, Hiroshima-shi, Hiroshima 734-8553, Japan.
Motoyuki Sugai: Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjukuku, Tokyoto, Japan.
Hitoshi Komatsuzawa: Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, 1-2-3,Kasumi,Minamiku, Hiroshima-shi, Hiroshima 734-8553, Japan.
Bacteriophages are expected to be therapeutic agents against infectious diseases. are involved in dental plaque formation related to dental caries and periodontitis. In , lytic bacteriophages have been isolated previously, but the isolation of temperate bacteriophage has not been reported although their presence in the genome has been confirmed. Here, we report the isolation of temperate bacteriophage, φKSM96, from . φKSM96 has a circular DNA 39,820 bp long and reveals Siphoviridae morphology. φKSM96 shows a broad range of susceptibility against strains with different serotypes. By the addition of φKSM96, growth and biofilm formation were significantly inhibited. In cocultures of with other bacterial species, the proportion of significantly decreased in the presence of φKSM96. In summary, φKSM96 shows selective anti- activity. The isolation of temperate bacteriophage is important for future genetic manipulation to create more efficient bacteriophages.
