Entomotherapy as an alternative treatment for diseases due to Gram-negative bacteria in Burkina Faso.
Mamadou Ouango, Hama Cissé, Rahim Romba, Samuel Fogné Drabo, Rasmané Semdé, Aly Savadogo, Olivier Gnankiné
Author Information
Mamadou Ouango: Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph KI ZERBO, 03 BP 7021, Ouagadougou, Burkina Faso. ouangovichy@gmail.com.
Hama Cissé: Laboratoire de Biochimie et Immunologie Appliquées, Université Joseph KI ZERBO, 03 BP 7021, Ouagadougou, Burkina Faso.
Rahim Romba: Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph KI ZERBO, 03 BP 7021, Ouagadougou, Burkina Faso.
Samuel Fogné Drabo: Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph KI ZERBO, 03 BP 7021, Ouagadougou, Burkina Faso.
Rasmané Semdé: Laboratoire du Développement du Médicament, Centre de Formation, de Recherche et d'Expertise en Sciences du Médicament, Université Joseph KI ZERBO, 03 BP 7021, Ouagadougou, Burkina Faso.
Aly Savadogo: Laboratoire de Biochimie et Immunologie Appliquées, Université Joseph KI ZERBO, 03 BP 7021, Ouagadougou, Burkina Faso.
Olivier Gnankiné: Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph KI ZERBO, 03 BP 7021, Ouagadougou, Burkina Faso.
Insects are known for their harmful effects. However, they also benefit humans, animals, plants, and ecosystems. Its beneficial uses include entomophagy and entomotherapy. This study aimed to evaluate the antibacterial activity of insect extracts against Gram-negative bacteria. Antibacterial activities of thirteen crude extracts of medicinal insects were tested against twelve Gram-negative bacteria by diffusion on agar. imipenem was used as an antibiotic for positive control. The thirteen extracts acted differently against certain Gram-negative bacteria. The largest inhibition diameter was for extracts of Cirina butyrospermi and Mylabris variabilis against Pseudomonas aeruginosa ATCC27853 and Salmonella enteritidis ATCC13076, respectively. The diameters of inhibition obtained using imipenem against these same bacterial strains were 13.0 ± 0.0 mm and 22 ± 1.0 mm, respectively. The lowest inhibition diameter (7.5 ± 0.0 mm) was obtained using Anopheles gambiae extract against Salmonella Typhimurium ATCC14028. imipenem was active on all strains tested. The highest values of the index multi-resistance to insect's extracts were reported for Pseudomonas aeruginosa ATCC9027 and Serratia odorifera 652411. Overall, the results of this study confirmed the antibacterial activities of insects used by traditional health practitioners to treat different pathologies. Entomotherapy could be an alternative treatment for certain infectious pathologies caused by gram-negative bacteria.
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