Combination Analysis of and DNA Methylation in Bronchial Washing Fluid for Lung Cancer Diagnosis.
Se Jin Park, Daeun Kang, Minhyeok Lee, Su Yel Lee, Young Gyu Park, TaeJeong Oh, Seunghyun Jang, Wan Jin Hwang, Sun Jung Kwon, Sungwhan An, Ji Woong Son, In Beom Jeong
Author Information
Se Jin Park: Division of Pulmonology, Department of Internal Medicine, Konyang University Hospital, Daejeon, Korea. ORCID
Daeun Kang: Division of Pulmonology, Department of Internal Medicine, Konyang University Hospital, Daejeon, Korea. ORCID
Minhyeok Lee: Division of Pulmonology, Department of Internal Medicine, Konyang University Hospital, Daejeon, Korea. ORCID
Su Yel Lee: Myunggok Medical Research Institute, College of Medicine, Konyang University, Daejeon, Korea. ORCID
Young Gyu Park: Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. ORCID
TaeJeong Oh: Genomictree, Inc., Daejeon, Korea. ORCID
Seunghyun Jang: Genomictree, Inc., Daejeon, Korea. ORCID
Wan Jin Hwang: Department of Thoracic and Cardiovascular Surgery, Konyang University Hospital, Daejeon, Korea. ORCID
Sun Jung Kwon: Division of Pulmonology, Department of Internal Medicine, Konyang University Hospital, Daejeon, Korea. ORCID
Sungwhan An: Genomictree, Inc., Daejeon, Korea. ORCID
Ji Woong Son: Division of Pulmonology, Department of Internal Medicine, Konyang University Hospital, Daejeon, Korea. sk1609@kyuh.ac.kr. ORCID
In Beom Jeong: Division of Pulmonology, Department of Internal Medicine, Konyang University Hospital, Daejeon, Korea. sharpion@kyuh.ac.kr. ORCID
BACKGROUND: When suspicious lesions are observed on computer-tomography (CT), invasive tests are needed to confirm lung cancer. Compared with other procedures, bronchoscopy has fewer complications. However, the sensitivity of peripheral lesion through bronchoscopy including washing cytology is low. A new test with higher sensitivity through bronchoscopy is needed. In our previous study, DNA methylation of in bronchial washing cytology has a diagnostic value for lung cancer. In this study, combination of and methylation obtained through bronchial washing cytology was evaluated as a diagnostic tool for lung cancer. METHODS: A total of 187 patients who had suspicious lesions in CT were enrolled. methylation test, methylation test, and cytological examination were performed using 3-plex LTE-qMSP test. RESULTS: Sixty-two patients were diagnosed with benign diseases and 125 patients were diagnosed with lung cancer. The sensitivity of was 74.4% and the specificity of was 91.9% respectively. methylation test had a sensitivity of 57.6% and a specificity of 96.8%. The combination of both methylation test and methylation test showed a sensitivity of 77.6% and a specificity of 90.3%. The sensitivity of lung cancer diagnosis was increased by combining both and methylation tests. CONCLUSION: Checking DNA methylation of both and genes using bronchial washing fluid can reduce the invasive procedure to diagnose lung cancer.
