Shaodong Xie: Department of Rehabilitation Medicine, Foshan Hospital of Traditional Chinese Medicine, Foshan, 528000, People's Republic of China.
Deming Zeng: Department of Rehabilitation Medicine, Foshan Hospital of Traditional Chinese Medicine, Foshan, 528000, People's Republic of China.
Hanwen Luo: Department of Rehabilitation Medicine, Foshan Hospital of Traditional Chinese Medicine, Foshan, 528000, People's Republic of China.
Ping Zhong: Department of Rehabilitation Medicine, Foshan Hospital of Traditional Chinese Medicine, Foshan, 528000, People's Republic of China.
Yu Wang: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, People's Republic of China. wydna@ciac.ac.cn.
Zhiqiang Xu: Department of Rehabilitation Medicine, Foshan Hospital of Traditional Chinese Medicine, Foshan, 528000, People's Republic of China. xuzq@fshtcm.com.cn.
Peibiao Zhang: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, People's Republic of China.
Electrical stimulation (ES) plays an important role in regulating cell osteoblast differentiation. As a noninvasive rehabilitation therapy method, Es has a unique role in postoperative recovery. Bone morphogenetic protein-2 (BMP-2) is the most commonly used bioactive molecule in in situ tissue engineering scaffolds, and it plays an important regulatory role in the whole process of bone injury repair. In this study, the osteogenic regulation of MC-3T3-E1 cells was studied by combining pulsed electrical stimulation (PES) and different concentrations of BMP-2. The results showed that PES and BMP-2 could synergically promote the proliferation of MC-3T3-E1 cells. The qPCR results of osteoblast-related genes showed that PES was synergistic with BMP-2 to promote osteoblast differentiation mainly through the regulation of the Smad/BMP and insulin like growth factor 1 (IGF1) signaling pathways. The expression level of alkaline phosphatase (ALP) and alizarin red staining further demonstrated the synergistic effect of PES and BMP-2 on promoting osteogenic differentiation and mineralization of cells. PES and BMP-2 could also synergically promote cell proliferation, expression of collagen I (COL-I) and ALP, and cell mineralization on the 3D-printed polylactic acid scaffold. These results suggest that the use of PES can enhance the osteogenic effect of in situ bone repair scaffolds containing BMP-2, reduce the dose of BMP-2 alone, and reduce the possible side effects of high-dose BMP-2 in vivo.
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Grants
202100043/Dengfeng Project of Foshan Hospital of Development Program
20200404110YY/Jilin Scientific and Technological Development Program
2022SYHZ0022/Science and Technology Cooperation Project between Jilin Province and Chinese Academy of Science
