An improved pathway for autonomous bioluminescence imaging in eukaryotes.
Ekaterina S Shakhova, Tatiana A Karataeva, Nadezhda M Markina, Tatiana Mitiouchkina, Kseniia A Palkina, Maxim M Perfilov, Monika G Wood, Trish T Hoang, Mary P Hall, Liliia I Fakhranurova, Anna E Alekberova, Alena K Malyshevskaia, Dmitry A Gorbachev, Evgenia N Bugaeva, Ludmila K Pletneva, Vladislav V Babenko, Daria I Boldyreva, Andrey Y Gorokhovatsky, Anastasia V Balakireva, Feng Gao, Vladimir V Choob, Lance P Encell, Keith V Wood, Ilia V Yampolsky, Karen S Sarkisyan, Alexander S Mishin
Author Information
Ekaterina S Shakhova: Planta LLC, Moscow, Russia. ORCID
Tatiana A Karataeva: Planta LLC, Moscow, Russia.
Nadezhda M Markina: Planta LLC, Moscow, Russia.
Tatiana Mitiouchkina: Planta LLC, Moscow, Russia.
Kseniia A Palkina: Planta LLC, Moscow, Russia.
Maxim M Perfilov: Planta LLC, Moscow, Russia. ORCID
Monika G Wood: Promega Corporation, Madison, WI, USA. ORCID
Trish T Hoang: Promega Corporation, Madison, WI, USA.
Mary P Hall: Promega Corporation, Madison, WI, USA.
Liliia I Fakhranurova: Planta LLC, Moscow, Russia.
Anna E Alekberova: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Alena K Malyshevskaia: Planta LLC, Moscow, Russia.
Dmitry A Gorbachev: Planta LLC, Moscow, Russia.
Evgenia N Bugaeva: Planta LLC, Moscow, Russia.
Ludmila K Pletneva: Planta LLC, Moscow, Russia.
Vladislav V Babenko: Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.
Daria I Boldyreva: Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.
Andrey Y Gorokhovatsky: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Anastasia V Balakireva: Planta LLC, Moscow, Russia.
Feng Gao: Synthetic Biology Group, MRC Laboratory of Medical Sciences, London, UK.
Vladimir V Choob: Planta LLC, Moscow, Russia.
Lance P Encell: Promega Corporation, Madison, WI, USA.
Keith V Wood: Light Bio Inc, Ketchum, ID, USA.
Ilia V Yampolsky: Planta LLC, Moscow, Russia. ORCID
Karen S Sarkisyan: Planta LLC, Moscow, Russia. karen@light.bio. ORCID
Alexander S Mishin: Planta LLC, Moscow, Russia. alexander@planta.bio. ORCID
The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.
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Grants
UKRI MC-A658-5QEA0/RCUK | Medical Research Council (MRC)
