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Molecular biology reports
Jan 25, 2024;51 (1): 192.

Exploring virulence factors of Helicobacter pylori isolated from gastric biopsy.

Parisa Javanbakhat, Hadi Peeridogaheh, Rasool Nemati, Abbas Yazdanbod, Amir Teimourpour, Mahin Sadeghnezhad, Majid Esmaelizad, Roghayeh Teimourpour
Author Information
  1. Parisa Javanbakhat: Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
  2. Hadi Peeridogaheh: Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. h.peeridogaheh@arums.ac.ir.
  3. Rasool Nemati: Departments of Internal Medicine, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
  4. Abbas Yazdanbod: Departments of Internal Medicine, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
  5. Amir Teimourpour: Blood Transfusion Research Center, High Institute for Research and Education, Tehran, Iran.
  6. Mahin Sadeghnezhad: Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
  7. Majid Esmaelizad: Central Lab, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  8. Roghayeh Teimourpour: Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. r.teymourpour@gmail.com.
PMID: 38270789 DOI: 10.1007/s11033-023-09075-z

Abstract

BACKGROUND: Helicobacter pylori (H. pylori) colonizes human gastric mucosa and is classified as class one carcinogenic bacteria. In this regard, this study aimed to detect major virulence factors in H. pylori strains recovered from gastric biopsy in patients referred to Aras Clinique in Ardabil, northwest of Iran (2019-2021).
MATERIALS AND METHODS: In this descriptive-cross sectional study, 287 dyspeptic patients were included. For bacterial isolation, gastric biopsy specimens (n=287) were taken from gastric antrum, then aseptically were cultured on the selective medium and incubated at 37C in microaerophilic conditions for 3-5 days.
RESULTS: 25.18% of all (n = 70) patients were found to be infected with H. pylori upon endoscopy. Of them, 9 patients (12.857%) and 2 patients (2.875%) had peptic ulcer disease and gastric cancer respectively. According to the different patterns of virulence factors, 57 virutypes were identified in which oipA-vacAs1-vacAm2 (3, 4.28% n =) and oipA-vacAs1-vacAs2-vacAm2 (3, 4.28% n =) were the most common patterns. The simultaneous presence of vacAS2, vacAm2 and hopQ2 genes was observed in both patients with gastric cancer. OipA (n = 562.5%), VacAs1 (n = 6.75%), VacAs2 (n = 6.75%), and VacAm2 (n = 787.5%) were found to be the most prevalent virulence factor.
CONCLUSION: According previous studies, it is confirmed that the cagPAI gene cluster and vacA gene alleles are strongly correlated with gastritis and gastrointestinal tract adenocarcinomas. Our study indicated that 50% of the indigenous strains of H. pylori harbor these oncogenic genes and they are hypervirulent.

Keywords

Gastric biopsy Gastric cancer H. pylori Peptic ulcer Urease

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Grants

  1. 072/Ardabil University of Medical Sciences

MeSH Term

Humans
Helicobacter pylori
Stomach Neoplasms
Cross-Sectional Studies
Biopsy
Pyloric Antrum
Journal Article

Word Cloud

Created with Highcharts 10.0.0pylorigastricpatientsHvirulencebiopsystudyfactorscancerHelicobacterstrainsfound2ulcerAccordingpatterns3428%n =genes5%n = 675%geneGastricBACKGROUND:colonizeshumanmucosaclassifiedclassonecarcinogenicbacteriaregardaimeddetectmajorrecoveredreferredArasCliniqueArdabilnorthwestIran2019-2021MATERIALSANDMETHODS:descriptive-crosssectional287dyspepticincludedbacterialisolationspecimensn=287takenantrumasepticallyculturedselectivemediumincubated37Cmicroaerophilicconditions3-5daysRESULTS:2518%n = 70infectedwith Hupon endoscopy912857%875%pepticdiseaserespectivelydifferent57virutypesidentifiedoipA-vacAs1-vacAm2oipA-vacAs1-vacAs2-vacAm2commonsimultaneouspresencevacAS2vacAm2hopQ2observedOipAn = 562VacAs1VacAs2VacAm2n = 787prevalentfactorCONCLUSION:previousstudiesconfirmedcagPAIclustervacAallelesstronglycorrelatedgastritisgastrointestinaltractadenocarcinomasindicated50%indigenousharboroncogenichypervirulentExploringisolatedPepticUrease

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