A QTL of eggplant shapes the rhizosphere bacterial community, co-responsible for resistance to bacterial wilt.
Chao Gong, Zhenshuo Wang, Zhiliang Li, Baojuan Sun, Wenlong Luo, Shanwei Luo, Shuting Chen, Peiting Mai, Zhenxing Li, Ye Li, Yikui Wang, Tao Li
Author Information
Chao Gong: Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Zhenshuo Wang: Department of Plant Pathology, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Zhiliang Li: Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Baojuan Sun: Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Wenlong Luo: Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Shanwei Luo: Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Shuting Chen: Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Peiting Mai: Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Zhenxing Li: Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Ye Li: Harbin Academy of Agricultural Sciences, Harbin, Heilongjiang, 150029, China.
Yikui Wang: Institute of Vegetable, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China.
Tao Li: Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangzhou, 510640, China.
Resistant crop cultivars can recruit beneficial rhizobacteria to resist disease. However, whether this recruitment is regulated by quantitative trait loci (QTL) is unclear. The role of QTL in recruiting specific bacteria against bacterial wilt (BW) is an important question of practical significance to disease management. Here, to identify QTL controlling BW resistance, Super-BSA was performed in F plants derived from resistant eggplant cultivar R06112 × susceptible cultivar S55193. The QTL was narrowed down through BCF-BCF individuals by wilting symptoms and KASP markers. Rhizosphere bacterial composition of R06112, S55193, and resistant individuals EB158 (with the QTL) and susceptible individuals EB327 (without QTL) from BCF generation were assessed by Illumina sequencing-based analysis, and the activation of plant immunity by the bacterial isolates was analyzed. Evidence showed that BW-resistant is controlled by one QTL located at the 270 kb region on chromosome 10, namely , and as candidate genes confirmed by RNA-Seq. has a significant effect on rhizobacteria composition and significantly recruits . pp. A SynCom of three isolated . pp trains significantly reduced the disease incidence, changed activities of CAT, PPO, and PAL and concentration of NO, HO, and O, activated SA and JA signaling-dependent ISR, and displayed immune activation against in eggplant. Our findings demonstrate for the first time that the QTL can recruit beneficial rhizobacteria, which jointly promote the suppression of BW. This method charts a path to develop the QTL in resistant cultivar-driven probiotics to ameliorate plant diseases.
