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Jan 25, 2024;22 (2):

Cold-Azurin, a New Antibiofilm Protein Produced by the Antarctic Marine Bacterium sp. TAE6080.

Caterina D'Angelo, Marika Trecca, Andrea Carpentieri, Marco Artini, Laura Selan, Maria Luisa Tutino, Rosanna Papa, Ermenegilda Parrilli
Author Information
  1. Caterina D'Angelo: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy. ORCID
  2. Marika Trecca: Department of Public Health and Infectious Diseases, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy.
  3. Andrea Carpentieri: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy. ORCID
  4. Marco Artini: Department of Public Health and Infectious Diseases, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy.
  5. Laura Selan: Department of Public Health and Infectious Diseases, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy.
  6. Maria Luisa Tutino: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy. ORCID
  7. Rosanna Papa: Department of Public Health and Infectious Diseases, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy. ORCID
  8. Ermenegilda Parrilli: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy. ORCID
PMID: 38393032 DOI: 10.3390/md22020061

Abstract

Biofilm is accountable for nosocomial infections and chronic illness, making it a serious economic and public health problem. , thanks to its ability to form biofilm and colonize biomaterials, represents the most frequent causative agent involved in biofilm-associated infections of medical devices. Therefore, the research of new molecules able to interfere with biofilm formation has a remarkable interest. In the present work, the attention was focused on sp. TAE6080, an Antarctic marine bacterium able to produce and secrete an effective antibiofilm compound. The molecule responsible for this activity was purified by an activity-guided approach and identified by LC-MS/MS. Results indicated the active protein was a periplasmic protein similar to the PAO1 azurin, named cold-azurin. The cold-azurin was recombinantly produced in and purified. The recombinant protein was able to impair attachment to the polystyrene surface and effectively prevent biofilm formation.

Keywords

Staphylococcus epidermidis antibiofilm azurin cold-adapted bacteria

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MeSH Term

Pseudomonas
Azurin
Anti-Bacterial Agents
Antarctic Regions
Escherichia coli
Chromatography, Liquid
Tandem Mass Spectrometry
Biofilms
Pseudomonas aeruginosa
Staphylococcus epidermidis

Chemicals

Azurin
Anti-Bacterial Agents
Journal Article

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