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Planta
Apr 18, 2024;259 (6): 125.

Cloning and functional identification of apple LATERAL ORGAN BOUNDARY DOMAIN 3 (LBD3) transcription factor in the regulation of drought and salt stress.

Yaqi Liu, Xiu-Hong An, Haofeng Liu, Tingting Zhang, Xiaowen Li, Ranxin Liu, Chang Li, Yi Tian, Chunxiang You, Xiao-Fei Wang
Author Information
  1. Yaqi Liu: Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China.
  2. Xiu-Hong An: National Engineering Research Center for Agriculture in Northern Mountainous Areas, Agricultural Technology Innovation Center in Mountainous Areas of Hebei Province, Hebei Agricultural University, Baoding, Hebei, China.
  3. Haofeng Liu: Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China.
  4. Tingting Zhang: Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China.
  5. Xiaowen Li: Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China.
  6. Ranxin Liu: Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China.
  7. Chang Li: Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China.
  8. Yi Tian: National Engineering Research Center for Agriculture in Northern Mountainous Areas, Agricultural Technology Innovation Center in Mountainous Areas of Hebei Province, Hebei Agricultural University, Baoding, Hebei, China.
  9. Chunxiang You: Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China. youchunxiang@sdau.edu.cn.
  10. Xiao-Fei Wang: Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018, Shandong, China. xfwang2004@163.com. ORCID
PMID: 38634979 DOI: 10.1007/s00425-024-04373-7

Abstract

MAIN CONCLUSION: Overexpression of MdLBD3 in Arabidopsis reduced sensitivity to salt and drought stresses and was instrumental in promoting early flowering. Salt and drought stresses have serious effects on plant growth. LATERAL ORGAN BOUNDARY DOMAIN (LBD) proteins are a plant-specific transcription factors (TFs) family and play important roles in plants in resisting to abiotic stress. However, about the function of LBDs in apple and other woody plants is little known. In this study, protein sequences of the LBD family TFs in apples were identified which contained conserved LOB domains. The qRT-PCR analysis showed that the MdLBD3 gene was widely expressed in various tissues and organs. The subcellular localization assay showed that the MdLBD3 protein was localized in the nucleus. Ectopic expression of MdLBD3 in Arabidopsis positively regulated its salt and drought resistance, and promoted early flowering. Collectively, these results showed that MdLBD3 improved the abiotic stress resistance, plant growth and development. Overall, this study provided a new gene for breeding that can increase the abiotic stress tolerance in apple.

Keywords

MdLBD3 Abiotic stress Early flowering Growth and development

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Grants

  1. 32272683/National Natural Science Foundation of China
  2. 2022TZXD008-02/the Shandong Province Key R&D Program of China
  3. CARS-27/the China Agriculture Research System of MOF and MARA

MeSH Term

Transcription Factors
Malus
Droughts
Arabidopsis
Plant Breeding
Salt Stress
Cloning, Molecular

Chemicals

Transcription Factors
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0MdLBD3stressdroughtsaltfloweringabioticappleshowedArabidopsisstressesearlyplantgrowthLATERALORGANBOUNDARYDOMAINLBDtranscriptionTFsfamilyplantsstudyproteingeneresistancedevelopmentMAINCONCLUSION:OverexpressionreducedsensitivityinstrumentalpromotingSaltseriouseffectsproteinsplant-specificfactorsplayimportantrolesresistingHoweverfunctionLBDswoodylittleknownsequencesapplesidentifiedcontainedconservedLOBdomainsqRT-PCRanalysiswidelyexpressedvarioustissuesorganssubcellularlocalizationassaylocalizednucleusEctopicexpressionpositivelyregulatedpromotedCollectivelyresultsimprovedOverallprovidednewbreedingcanincreasetoleranceCloningfunctionalidentification3LBD3factorregulationAbioticEarlyGrowth

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