The evaluation of Staphylococcus aureus and Staphylococcus epidermidis in hospital air, their antibiotic resistance and sensitivity of S. aureus to cefoxitin.

Mostafa Leili, Sedighe Afrasiabi, Roohollah Rostami, Mohammad Khazaei, Mahdaneh Roshani, Zahra Tarin
Author Information
  1. Mostafa Leili: Department of Environmental Health Engineering, School of Public Health and Research Center for Health Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.
  2. Sedighe Afrasiabi: Department of Environmental Health Engineering, School of Public Health, Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran. afraseyabi.00@gmail.com.
  3. Roohollah Rostami: Department of Environmental Health Engineering, Research Center for Health Sciences and Technologies, Semnan University of Medical Sciences, Semnan, Iran.
  4. Mohammad Khazaei: Department of Environmental Health Engineering, School of Public Health and Research Center for Health Sciences, Hamadan University of Medical Sciences, Hamadan, Iran.
  5. Mahdaneh Roshani: Department of Microbiology, Faculty of Medicine, Medical Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran.
  6. Zahra Tarin: Department of Occupational Health Engineering, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran.

Abstract

Staphylococci as a nosocomial infection agent, increases the possibility of contracting diseases such as wound infection, sepsis and skin infections in humans. It was shown that Staphylococcus aureus considered as a commensal organism causing various both endemic and epidemic hospital-acquired infections. Air samples were collected from Sina Hospital, Hamadan city, which dedicated to various respiratory diseases and analysed by biochemical tests. The resistance and sensitivity of bacterial strains to the cefoxitin antibiotic were also determined. Staphylococcus aureus density (CFU/m) were measured in the air of various wards as follows: infectious 13.35 ± 7.57, poisoning 29.84 ± 33.43, emergency 8.64 ± 2.72, eye operation room 0, recovery room 6.28 ± 4.90, skin outpatient operation room 4.71 ± 2.36, respiratory isolation 0, ICU 0.79 ± 1.36, and the administrative room 6.28 ± 5.93; while the Staphylococcus epidermidis were as follows: infectious 1.57 ± 2.35, poisoning 2.35 ± 4.08, emergency 2.35 ± 2.35, eye operation room 0, recovery room 0.78 ± 1.36, skin outpatient operation room 2.35 ± 2.35, respiratory isolation 0, ICU 2.35 ± 4.08, and the administrative room 1.57 ± 1.36. The positive and negative control samples showed a concentration of 0. Moreover, among the S. aureus isolates, 33.3% were found to be resistant to cefoxitin, while 40.6% showed to be sensitive. Based on the results, the number of active people and the type and quality of ventilation are very effective in the air quality of various wards of hospital. The poisoning section showed the most contaminated air and the highest resistance and sensitivity to the cefoxitin antibiotic.

Keywords

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Grants

  1. (Grant No. 140104142673, by Hamadan University of Medical Sciences.)/This manuscript is a part of a funded MSc. thesis (Grant No. 140104142673, by Hamadan University of Medical Sciences.)

MeSH Term

Staphylococcus aureus
Staphylococcus epidermidis
Cefoxitin
Anti-Bacterial Agents
Humans
Air Microbiology
Hospitals
Microbial Sensitivity Tests
Cross Infection
Drug Resistance, Bacterial
Staphylococcal Infections

Chemicals

Cefoxitin
Anti-Bacterial Agents

Word Cloud

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