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Current microbiology
Apr 23, 2024;81 (6): 154.

Extent of Virulence and Antibiotic Resistance Genes in Helicobacter pylori and Campylobacteria.

R Shyama Prasad Rao, Sudeep D Ghate, Larina Pinto, Prashanth Suravajhala, Prakash Patil, Praveenkumar Shetty, Nagib Ahsan
Author Information
  1. R Shyama Prasad Rao: Center for Bioinformatics, NITTE Deemed to be University, Mangaluru, 575018, India. shyamaprasad.rao@nitte.edu.in. ORCID
  2. Sudeep D Ghate: Center for Bioinformatics, NITTE Deemed to be University, Mangaluru, 575018, India. sudeep.ghate@nitte.edu.in. ORCID
  3. Larina Pinto: Center for Bioinformatics, NITTE Deemed to be University, Mangaluru, 575018, India. ORCID
  4. Prashanth Suravajhala: Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Clappana, Kerala, 690525, India. ORCID
  5. Prakash Patil: Central Research Laboratory, KS Hegde Medical Academy (KSHEMA), NITTE Deemed to be University, Mangaluru, 575018, India. ORCID
  6. Praveenkumar Shetty: Central Research Laboratory, KS Hegde Medical Academy (KSHEMA), NITTE Deemed to be University, Mangaluru, 575018, India. ORCID
  7. Nagib Ahsan: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, USA. ORCID
PMID: 38652129 DOI: 10.1007/s00284-024-03653-5

Abstract

Helicobacter pylori, a member of the clade campylobacteria, is the leading cause of chronic gastritis and gastric cancer. Virulence and antibiotic resistance of H. pylori are of great concern to public health. However, the relationship between virulence and antibiotic resistance genes in H. pylori in relation to other campylobacteria remains unclear. Using the virulence and comprehensive antibiotic resistance databases, we explored all available 354 complete genomes of H. pylori and compared it with 90 species of campylobacteria for virulence and antibiotic resistance genes/proteins. On average, H. pylori had 129 virulence genes, highest among Helicobacter spp. and 71 antibiotic resistance genes, one of the lowest among campylobacteria. Just 2.6% of virulence genes were shared by all campylobacterial members, whereas 9.4% were unique to H. pylori. The cytotoxin-associated genes (cags) seemed to be exclusive to H. pylori. Majority of the isolates from Asia and South America were cag2-negative and many antibiotic resistance genes showed isolate-specific patterns of occurrence. Just 15 (8.8%) antibiotic resistance genes, but 103 (66%) virulence genes including 25 cags were proteomically identified in H. pylori. Arcobacterial members showed large variation in the number of antibiotic resistance genes and there was a positive relation with the genome size. Large repository of antibiotic resistance genes in campylobacteria and a unique set of virulence genes might have important implications in shaping the course of virulence and antibiotic resistance in H. pylori.

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MeSH Term

Helicobacter pylori
Drug Resistance, Bacterial
Anti-Bacterial Agents
Virulence
Virulence Factors
Bacterial Proteins
Genome, Bacterial
Helicobacter Infections
Humans

Chemicals

Anti-Bacterial Agents
Virulence Factors
Bacterial Proteins
Journal Article

Word Cloud

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