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Nature communications
May 17, 2024;15 (1): 4200.

The matrisome landscape controlling in vivo germ cell fates.

Aqilah Amran, Lara Pigatto, Johanna Farley, Rasoul Godini, Roger Pocock, Sandeep Gopal
Author Information
  1. Aqilah Amran: Department of Experimental Medical Science, Lund University, Lund, Sweden.
  2. Lara Pigatto: Department of Experimental Medical Science, Lund University, Lund, Sweden.
  3. Johanna Farley: Department of Experimental Medical Science, Lund University, Lund, Sweden. ORCID
  4. Rasoul Godini: Development and Stem Cells Program, Monash Biomedicine Discovery Institute. Department of Anatomy and Developmental Biology, Monash University, Melbourne, VIC, Australia.
  5. Roger Pocock: Development and Stem Cells Program, Monash Biomedicine Discovery Institute. Department of Anatomy and Developmental Biology, Monash University, Melbourne, VIC, Australia. roger.pocock@monash.edu. ORCID
  6. Sandeep Gopal: Department of Experimental Medical Science, Lund University, Lund, Sweden. sandeep.gopal@med.lu.se. ORCID
PMID: 38760342 DOI: 10.1038/s41467-024-48283-4

Abstract

The developmental fate of cells is regulated by intrinsic factors and the extracellular environment. The extracellular matrix (matrisome) delivers chemical and mechanical cues that can modify cellular development. However, comprehensive understanding of how matrisome factors control cells in vivo is lacking. Here we show that specific matrisome factors act individually and collectively to control germ cell development. Surveying development of undifferentiated germline stem cells through to mature oocytes in the Caenorhabditis elegans germ line enabled holistic functional analysis of 443 conserved matrisome-coding genes. Using high-content imaging, 3D reconstruction, and cell behavior analysis, we identify 321 matrisome genes that impact germ cell development, the majority of which (>80%) are undescribed. Our analysis identifies key matrisome networks acting autonomously and non-autonomously to coordinate germ cell behavior. Further, our results demonstrate that germ cell development requires continual remodeling of the matrisome landscape. Together, this study provides a comprehensive platform for deciphering how extracellular signaling controls cellular development and anticipate this will establish new opportunities for manipulating cell fates.

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Grants

  1. 2019-02020/Vetenskapsr��det (Swedish Research Council)
  2. 22 2125 Pj/Cancerfonden (Swedish Cancer Society)
  3. N/A/Franke och Margareta Bergqvists Stiftelse f��r Fr��mjande av Cancerforskning
  4. 20200545/Crafoordska Stiftelsen (Crafoord Foundation)
  5. N/A/Kungliga Fysiografiska S��llskapet i Lund (Royal Physiographic Society in Lund)
  6. DE190100174/Department of Education and Training | Australian Research Council (ARC)
  7. DP200103293/Department of Education and Training | Australian Research Council (ARC)
  8. GNT1161439/Department of Health | National Health and Medical Research Council (NHMRC)
  9. GNT1105374/Department of Health | National Health and Medical Research Council (NHMRC)
  10. GNT1137645/Department of Health | National Health and Medical Research Council (NHMRC)
  11. 2018825/Department of Health | National Health and Medical Research Council (NHMRC)

MeSH Term

Animals
Caenorhabditis elegans
Extracellular Matrix
Germ Cells
Cell Differentiation
Caenorhabditis elegans Proteins
Gene Expression Regulation, Developmental
Signal Transduction
Cell Lineage
Oocytes
Journal Article
Article has an altmetric score of 11

Word Cloud

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