Effect of baicalin on eradicating biofilms of bovine milk derived Acinetobacter lwoffii.

Chengjun Ma, Cui Mei, JingJing Liu, Hui Li, Min Jiao, Huiming Hu, Yang Zhang, Jing Xiong, Yuzhang He, Wei Wei, Hongzao Yang, Hongwei Chen
Author Information
  1. Chengjun Ma: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  2. Cui Mei: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  3. JingJing Liu: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  4. Hui Li: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  5. Min Jiao: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  6. Huiming Hu: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  7. Yang Zhang: National Center of Technology Innovation for Pigs, Chongqing, 402460, China.
  8. Jing Xiong: National Center of Technology Innovation for Pigs, Chongqing, 402460, China.
  9. Yuzhang He: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  10. Wei Wei: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  11. Hongzao Yang: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China. yhz03008@swu.edu.cn.
  12. Hongwei Chen: College of Veterinary Medicine, Southwest University, Chongqing, 402460, China. dyxchw@swu.edu.cn.

Abstract

BACKGROUND: Acinetobacter lwoffii (A.lwoffii) is a serious zoonotic pathogen that has been identified as a cause of infections such as meningitis, bacteremia and pneumonia. In recent years, the infection rate and detection rate of A.lwoffii is increasing, especially in the breeding industry. Due to the presence of biofilms, it is difficult to eradicate and has become a potential super drug-resistant bacteria. Therefore, eradication of preformed biofilm is an alternative therapeutic action to control A.lwoffii infection. The present study aimed to clarify that baicalin could eradicate A.lwoffii biofilm in dairy cows, and to explore the mechanism of baicalin eradicating A.lwoffii.
RESULTS: The results showed that compared to the control group, the 4 MIC of baicalin significantly eradicated the preformed biofilm, and the effect was stable at this concentration, the number of viable bacteria in the biofilm was decreased by 0.67 LogCFU/mL. The total fluorescence intensity of biofilm bacteria decreased significantly, with a reduction rate of 67.0%. There were 833 differentially expressed genes (367 up-regulated and 466 down-regulated), whose functions mainly focused on oxidative phosphorylation, biofilm regulation system and trehalose synthesis. Molecular docking analysis predicted 11 groups of target proteins that were well combined with baicalin, and the content of trehalose decreased significantly after the biofilm of A.lwoffii was treated with baicalin.
CONCLUSIONS: The present study evaluated the antibiofilm potential of baicalin against A.lwoffii. Baicalin revealed strong antibiofilm potential against A.lwoffii. Baicalin induced biofilm eradication may be related to oxidative phosphorylation and TCSs. Moreover, the decrease of trehalose content may be related to biofilm eradication.

Keywords

References

  1. World J Microbiol Biotechnol. 2017 Mar;33(3):50 [PMID: 28188589]
  2. Environ Microbiol. 2017 Dec;19(12):5088-5099 [PMID: 29124876]
  3. Nat Rev Microbiol. 2020 Oct;18(10):571-586 [PMID: 32533131]
  4. Nat Rev Microbiol. 2023 May;21(5):280-295 [PMID: 36411397]
  5. NPJ Biofilms Microbiomes. 2021 Jan 25;7(1):8 [PMID: 33495449]
  6. Infect Drug Resist. 2023 May 09;16:2861-2882 [PMID: 37193303]
  7. J Pharm Biomed Anal. 2014 Jan;87:241-60 [PMID: 23642848]
  8. Int J Food Microbiol. 2023 Jun 2;394:110165 [PMID: 36933360]
  9. Biochem Biophys Res Commun. 2022 Sep 17;621:130-136 [PMID: 35820283]
  10. Environ Microbiol. 2020 Dec;22(12):5156-5166 [PMID: 32618111]
  11. Microb Pathog. 2020 Dec;149:104514 [PMID: 32976967]
  12. Microb Pathog. 2015 Oct;87:21-9 [PMID: 26169236]
  13. Biology (Basel). 2021 Sep 04;10(9): [PMID: 34571748]
  14. Pharmacol Ther. 2002 Nov-Dec;96(2-3):67-202 [PMID: 12453566]
  15. Biofouling. 2019 Feb;35(2):143-158 [PMID: 30884970]
  16. J Hazard Mater. 2023 Aug 5;455:131587 [PMID: 37172383]
  17. J Bacteriol. 2018 Dec 7;201(1): [PMID: 30322852]
  18. Front Microbiol. 2019 Aug 07;10:1837 [PMID: 31456771]
  19. Int J Antimicrob Agents. 2018 Jul;52(1):35-41 [PMID: 29530588]
  20. Nat Microbiol. 2021 Feb;6(2):151-156 [PMID: 33398098]
  21. ACS Infect Dis. 2021 Aug 13;7(8):2116-2126 [PMID: 34105954]
  22. Vet Res. 2022 Oct 8;53(1):80 [PMID: 36209206]
  23. BMC Oral Health. 2020 Apr 22;20(1):123 [PMID: 32321490]
  24. Vet Microbiol. 2021 Nov;262:109242 [PMID: 34562786]
  25. Microb Pathog. 2021 Mar;152:104660 [PMID: 33253855]
  26. Molecules. 2021 Mar 10;26(6): [PMID: 33801847]
  27. Microbiol Spectr. 2022 Dec 21;10(6):e0299722 [PMID: 36374018]
  28. BMC Microbiol. 2017 Mar 8;17(1):56 [PMID: 28274217]
  29. Front Microbiol. 2021 Sep 10;12:725755 [PMID: 34566929]
  30. J Bacteriol. 2020 Sep 23;202(20): [PMID: 32967911]
  31. Sci Rep. 2018 Jul 11;8(1):10446 [PMID: 29993029]
  32. J Dairy Sci. 2018 Mar;101(3):2415-2422 [PMID: 29290430]
  33. Bioorg Chem. 2019 Jun;87:291-301 [PMID: 30913464]
  34. Biochim Biophys Acta Mol Cell Res. 2023 Feb;1870(2):119403 [PMID: 36427551]
  35. Clin Exp Allergy. 2022 Apr;52(4):518-529 [PMID: 34874580]
  36. Sci Rep. 2014 Jun 27;4:5467 [PMID: 24970710]
  37. mSystems. 2021 Feb 16;6(1): [PMID: 33594002]
  38. Mol Inform. 2015 Aug;34(8):513-25 [PMID: 27490497]
  39. Methods Mol Biol. 2014;1147:175-86 [PMID: 24664833]
  40. RSC Adv. 2022 Mar 21;12(14):8737-8742 [PMID: 35424809]
  41. Evid Based Complement Alternat Med. 2013;2013:987326 [PMID: 23606893]
  42. World J Microbiol Biotechnol. 2022 Jan 22;38(3):44 [PMID: 35064842]
  43. ACS Appl Mater Interfaces. 2020 Mar 25;12(12):13731-13738 [PMID: 32155326]
  44. Curr Microbiol. 2023 Mar 6;80(4):126 [PMID: 36877407]
  45. Transbound Emerg Dis. 2018 Dec;65(6):1816-1822 [PMID: 30239149]
  46. Front Vet Sci. 2022 Jul 12;9:945491 [PMID: 35903134]
  47. PLoS One. 2017 Apr 28;12(4):e0176883 [PMID: 28453568]
  48. PLoS One. 2014 Jan 31;9(1):e87312 [PMID: 24498070]
  49. Microorganisms. 2021 Feb 14;9(2): [PMID: 33672820]

Grants

  1. 202310635087/National Training Program of Innovation and Entrepreneurship for Undergraduates
  2. SWU-KQ22045/Fundamental Research Funds for Central Universities
  3. CSTB2023TIAD-LDX0006/Chongqing Technical Innovation and Application Development Special General Project
  4. NCTIP-XD/B12, NCTIP-XD/C17/National Center of Technology Innovation for Pigs
  5. 022LYXZ030/the Project of Shandong Province on the Transformation of Scientific and Technological Achievements

MeSH Term

Biofilms
Animals
Flavonoids
Acinetobacter
Cattle
Milk
Anti-Bacterial Agents
Microbial Sensitivity Tests
Molecular Docking Simulation
Female
Acinetobacter Infections

Chemicals

baicalin

Word Cloud

Created with Highcharts 10.0.0lwoffiibiofilmAbaicalinratepotentialbacteriaeradicationsignificantlydecreasedtrehaloseBaicalinAcinetobacterinfectioneradicatepreformedcontrolpresentstudy67oxidativephosphorylationcontentantibiofilmmayrelatedBACKGROUND:seriouszoonoticpathogenidentifiedcauseinfectionsmeningitisbacteremiapneumoniarecentyearsdetectionincreasingespeciallybreedingindustryDuepresencebiofilmsdifficultbecomesuperdrug-resistantThereforealternativetherapeuticactionaimedclarifydairycowsexploremechanismeradicatingRESULTS:resultsshowedcomparedgroup4MICeradicatedeffectstableconcentrationnumberviable0LogCFU/mLtotalfluorescenceintensityreduction0%833differentiallyexpressedgenes367up-regulated466down-regulatedwhosefunctionsmainlyfocusedregulationsystemsynthesis Moleculardockinganalysispredicted11groupstargetproteinswellcombinedtreatedCONCLUSIONS:evaluatedrevealedstronginducedTCSsMoreoverdecreaseEffect of baicalin on eradicating biofilms of bovine milk derived Acinetobacter lwoffiiBiofilmEradicationTrehalose

Similar Articles

Cited By