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TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
May 21, 2024;137 (6): 137.

Identification and functional marker development of SbPLSH1 conferring purple leaf sheath in sorghum.

Puyuan Yang, Yuzhe Bai, Dongting Zhao, Jianghui Cui, Weiping Yang, Yukun Gao, Jiandong Zhang, Zhibo Wang, Meng Wang, Wei Xue, Jinhua Chang
Author Information
  1. Puyuan Yang: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
  2. Yuzhe Bai: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
  3. Dongting Zhao: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
  4. Jianghui Cui: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
  5. Weiping Yang: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
  6. Yukun Gao: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
  7. Jiandong Zhang: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
  8. Zhibo Wang: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
  9. Meng Wang: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
  10. Wei Xue: Baoding Vocational and Technical College, Baoding, 071000, China.
  11. Jinhua Chang: College of Agronomy, Hebei Agricultural University, Baoding, 071000, China. jhchang2006@126.com. ORCID
PMID: 38769163 DOI: 10.1007/s00122-024-04623-y

Abstract

KEY MESSAGE: We identified a SbPLSH1gene conferring purple leaf sheath in sorghum (sorghumbicolor(L.) Moench)and developed a functional markerfor it. The purple leaf sheath of sorghum, a trait mostly related to anthocyanin deposition, is a visually distinguishable morphological marker widely used to evaluate the purity of crop hybrids. We aimed to dissect the genetic mechanism for leaf sheath color to mine the genes regulating this trait. In this study, two F populations were constructed by crossing a purple leaf sheath inbred line (Gaoliangzhe) with two green leaf sheath inbred lines (BTx623 and Silimei). Based on the results of bulked-segregant analysis sequencing, bulk-segregant RNA sequencing, and map-based cloning, SbPLSH1 (Sobic.006G175700), which encodes a bHLH transcription factor on chromosome 6, was identified as the candidate gene for purple leaf sheath in sorghum. Genetic analysis demonstrated that overexpression of SbPLSH1 in Arabidopsis resulted in anthocyanin deposition and purple petiole, while two single-nucleotide polymorphism (SNP) variants on the exon 6 resulted in loss of function. Further haplotype analysis revealed that there were two missense mutations and one cis-acting element mutation in SbPLSH1, which are closely associated with leaf sheath color in sorghum. Based on the variations, a functional marker (LSC4-2) for marker-assisted selection was developed, which has a broad-spectrum capability of distinguishing leaf sheath color in natural variants. In summary, this study lays a foundation for analyzing the genetic mechanism for sorghum leaf sheath color.

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Grants

  1. 2019YFD1000703/National Key R&D Program of China
  2. 21326305D/Hebei Key Research & Development Program
  3. QN2021302/Hebei Province Science and Technology Research Youth Fund

MeSH Term

Sorghum
Plant Leaves
Polymorphism, Single Nucleotide
Anthocyanins
Genetic Markers
Phenotype
Pigmentation
Chromosome Mapping
Plant Proteins
Arabidopsis
Basic Helix-Loop-Helix Transcription Factors
Genes, Plant
Plants, Genetically Modified
Haplotypes
Gene Expression Regulation, Plant
Journal Article
Article has an altmetric score of 1

Word Cloud

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