Genomic insights into the conservation status of the Idle Crayfish Austropotamobius bihariensis Pârvulescu, 2019: low genetic diversity in the endemic crayfish species of the Apuseni Mountains.
Lena Bonassin, Lucian Pârvulescu, Ljudevit Luka Boštjančić, Caterina Francesconi, Judith Paetsch, Christelle Rutz, Odile Lecompte, Kathrin Theissinger
Author Information
Lena Bonassin: Department of Computer Science, Centre de Recherche en Biomédecine de Strasbourg, UMR 7357, University of Strasbourg, CNRS, Rue Eugène Boeckel 1, 67000, ICube, Strasbourg, France. ORCID
Lucian Pârvulescu: Department of Biology-Chemistry, Faculty of Chemistry, Biology, Geography, West University of Timisoara, Str. Pestalozzi 16A, 300115, Timisoara, Romania. lucian.parvulescu@e-uvt.ro. ORCID
Ljudevit Luka Boštjančić: Department of Computer Science, Centre de Recherche en Biomédecine de Strasbourg, UMR 7357, University of Strasbourg, CNRS, Rue Eugène Boeckel 1, 67000, ICube, Strasbourg, France. ORCID
Caterina Francesconi: LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberganlage 25, 60325, Frankfurt am Main, Germany. ORCID
Judith Paetsch: Department of Biogeography, University of Trier, Behringstraße 21, D-54296, Geozentrum, Trier, Germany.
Christelle Rutz: Department of Computer Science, Centre de Recherche en Biomédecine de Strasbourg, UMR 7357, University of Strasbourg, CNRS, Rue Eugène Boeckel 1, 67000, ICube, Strasbourg, France. ORCID
Odile Lecompte: Department of Computer Science, Centre de Recherche en Biomédecine de Strasbourg, UMR 7357, University of Strasbourg, CNRS, Rue Eugène Boeckel 1, 67000, ICube, Strasbourg, France. ORCID
Kathrin Theissinger: Institute for Insect Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 26, D-35392, Giessen, Germany. ORCID
BACKGROUND: Biodiversity in freshwater ecosystems is declining due to an increased anthropogenic footprint. Freshwater crayfish are keystone species in freshwater ecosystems and play a crucial role in shaping the structure and function of their habitats. The Idle Crayfish Austropotamobius bihariensis is a native European species with a narrow distribution range, endemic to the Apuseni Mountains (Romania). Although its area is small, the populations are anthropogenically fragmented. In this context, the assessment of its conservation status is timely. RESULTS: Using a reduced representation sequencing approach, we identified 4875 genomic SNPs from individuals belonging to 13 populations across the species distribution range. Subsequent population genomic analyses highlighted low heterozygosity levels, low number of private alleles and small effective population size. Our structuring analyses revealed that the genomic similarity of the populations is conserved within the river basins. CONCLUSION: Genomic SNPs represented excellent tools to gain insights into intraspecific genomic diversity and population structure of the Idle Crayfish. Our study highlighted that the analysed populations are at risk due to their limited genetic diversity, which makes them extremely vulnerable to environmental alterations. Thus, our results emphasize the need for conservation measures and can be used as a baseline to establish species management programs.
