Metagenomic sequencing combined with flow cytometry facilitated a novel microbial risk assessment framework for bacterial pathogens in municipal wastewater without cultivation.
Songzhe Fu, Rui Wang, Zheng Xu, Huiwen Zhou, Zhiguang Qiu, Lixin Shen, Qian Yang
Author Information
Songzhe Fu: Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education Dalian Ocean University Dalian China. ORCID
Rui Wang: Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education Dalian Ocean University Dalian China.
Zheng Xu: Shenzhen Yantian District People's Hospital Shenzhen China.
Huiwen Zhou: College of Life Science and Health Northeastern University Shenyang China.
Zhiguang Qiu: School of Environment and Energy, Shenzhen Graduate School Peking University Shenzhen China.
Lixin Shen: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education Northwest University Xi'an China.
Qian Yang: Center for Microbial Ecology and Technology (CMET) Ghent University Gent Belgium.
A workflow that combined metagenomic sequencing with flow cytometry was developed. The absolute abundance of pathogens was accurately estimated in mock communities and real samples. Metagenome-assembled genomes binned from metagenomic data set is robust in phylogenetic analysis and virulence profiling.
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