Next-generation sequencing reveals that miR-16-5p, miR-19a-3p, miR-451a, and miR-25-3p cargo in plasma extracellular vesicles differentiates sedentary young males from athletes.
Manuel Fernandez-Sanjurjo, Paola Pinto-Hernandez, Alberto D��valos, ��ngel Enrique D��az-Mart��nez, Roberto Mart��n-Hern��ndez, Juan Castilla-Silgado, Celia Toyos-Rodr��guez, Martin Whitham, Laura Amado-Rodr��guez, Guillermo Mu��iz-Albaiceta, Nicol��s Terrados, Benjam��n Fern��ndez-Garc��a, Eduardo Iglesias-Guti��rrez
Author Information
Manuel Fernandez-Sanjurjo: Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain. ORCID
Paola Pinto-Hernandez: Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain.
Alberto D��valos: Laboratory of Epigenetics of Lipid Metabolism, IMDEA Food Institute, CEI UAM + CSIC, Madrid, Spain.
��ngel Enrique D��az-Mart��nez: Clinical Laboratory, Sports Medicine Center, Spanish Sports Health Protection Agency, Spanish Government, Madrid, Spain.
Roberto Mart��n-Hern��ndez: Laboratory of Epigenetics of Lipid Metabolism, IMDEA Food Institute, CEI UAM + CSIC, Madrid, Spain.
Juan Castilla-Silgado: Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain.
Celia Toyos-Rodr��guez: NanoBioAnalysis Group, Department of Physical and Analytical Chemistry, University of Oviedo, Oviedo, Spain.
Martin Whitham: School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, UK.
Laura Amado-Rodr��guez: Instituto de Investigaci��n Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Guillermo Mu��iz-Albaiceta: Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain.
Nicol��s Terrados: Instituto de Investigaci��n Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Benjam��n Fern��ndez-Garc��a: Instituto de Investigaci��n Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Eduardo Iglesias-Guti��rrez: Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain.
A sedentary lifestyle and Olympic participation are contrary risk factors for global mortality and incidence of cancer and cardiovascular disease. Extracellular vesicle miRNAs have been described to respond to exercise. No molecular characterization of young male sedentary people versus athletes is available; so, our aim was to identify the extracellular vesicle miRNA profile of chronically trained young endurance and resistance male athletes compared to their sedentary counterparts. A descriptive case-control design was used with 16 sedentary young men, 16 Olympic male endurance athletes, and 16 Olympic male resistance athletes. Next-generation sequencing and RT-qPCR and external and internal validation were performed in order to analyze extracellular vesicle miRNA profiles. Endurance and resistance athletes had significant lower levels of miR-16-5p, miR-19a-3p, and miR-451a compared to sedentary people. Taking all together, exercise-trained miRNA profile in extracellular vesicles provides a differential signature of athletes irrespective of the type of exercise compared to sedentary people. Besides, miR-25-3p levels were specifically lower in endurance athletes which defines its role as a specific responder in this type of athletes. In silico analysis of this profile suggests a role in adaptive energy metabolism in this context that needs to be experimentally validated. Therefore, this study provides for the first time basal levels of circulating miRNA in extracellular vesicles emerge as relevant players in intertissue communication in response to chronic exercise exposure in young elite male athletes.
